نوع مقاله : مقاله پژوهشی

نویسنده

پژوهشکده‌ی کشاورزی هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، سازمان انرژی اتمی، صندوق پستی: 1498-31465، کرج- ایران

چکیده

ماهی آزاد دریای خزر (Salmo trutta caspius) گونه ماهی با ارزش دریای خزر و حوزه اطراف آن است که به ­عنوان گونه‎ای مناسب در صنعت شیلات کشورمان مطرح است. در این پژوهش تخم و اسپرم از ماهی آزاد دریای خزر استحصال شد. برای انجام ماده‎زایی (Gynogenesis) اسپرم با دزهای 450، 600 ،750، 900 و Gy 1050 توسط پرتو گاما (Co60) پرتودهی گردید. در مرحله بعد لقاح به ­صورت خشک با مخلوط نمودن تخمک و اسپرم‌­های پرتو دیده انجام شد. جهت القای پلوییدی حمام آبی با درجه حرارت‌­های 26 تا C˚28 برقرار گردید. نمونه ­گیری از باله دمی ماهی­‌های تیمارهای مختلف صورت گرفت و استخراج DNA صورت گرفته و تعیین جنسیت با استفاده از پرایمرهای 1S1E و 4AS2E مربوط به ژن SDY صورت پذیرفت. پس از رسیدن ماهیان آزاد به وزن مناسب با استفاده از بافت­ شناسی کلاسیک جنسیت مورد بررسی و مطالعه قرار گرفت. نتایج نشان داد که در گروهGy  900 میزان لقاح، بازماندگی لارو نسبت به سایر گروه­‌های گاینوژن با اختلاف معنی‌­داری بیش‌­تر بود (05/0p <). تعیین جنسیت با استفاده از روش­‌های مولکولی نشان داد که در تیمار ماده‎زایی شده با دز Gy 450 هنوز جنس نر (1 نمونه از 5 نمونه) وجود دارد؛ این در صورتی بود که در دزهای دیگر جنس نر وجود نداشت. اما در کلیه نمونه‌­های بافت­ شناسی نشان‌ه­ای از گناد جنسی نر مشاهده نمی‌­شود. با توجه به این نتایج می‌­توان نتیجه گرفت که ماده ­زایی ماهی آزاد دریای خزر با استفاده از روش پرتوتابی گاما به­ صورت موفقیت­ آمیزی انجام شده است و دز Gy 900 به ­عنوان دز مناسب برای استفاده از این روش در این گونه پیشنهاد می‌­شود.

کلیدواژه‌ها

عنوان مقاله [English]

Meiotic gynogenesis inducing in Caspian Sea salmon (Salmo trutta caspius) using gamma irradiation followed by sex determination using SDY gene

نویسنده [English]

  • Gh. Shahhosseini

Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, AEOI, P.O.Box: 31465-1498, Karaj - Iran

چکیده [English]

Caspian Sea salmon (Salmo trutta caspius) is a valuable fish species in the Caspian Sea and its surrounding basin, which is considered as a suitable species in our country's fishing industry. Caspian salmon ovums and sperms were extracted from Caspian salmon. To perform gynogenesis, the sperms were irradiated with a gamma ray (60Co) at doses of 450, 600, 750, 900, and 1050 Gy. In the next stage, fertilization was performed by mixing the ovum and the irradiated sperm. To induce ploidy, a water bath with a temperature of 26-28 ˚C was established. The tail fins of different treatments were sampled and the DNA was extracted and its sex was determined using primers E1S1 and E2AS4 related to SDY gene. After salmon reached the appropriate weight, its sex was studied using classical histology. The results showed that in the 900 g group of fertilization, the survival larval was significantly higher than other gynogen groups (p < 0.05). Gender determination using molecular methods showed that in the treatment of female with a dose of 450 Gy, there is still one male determined. (1 sample out of 5 samples), this happened in a case that there was no male in other doses. But in all histological samples, there is no sign of male gonad. According to these results, it can be concluded that the gynogenesis of Caspian Sea salmon has been done successfully using this gamma irradiation method and a dose of 900 Gy is recommended as a suitable dose for using this method in this species.

کلیدواژه‌ها [English]

  • Caspian sea salmon (Salmo trutta caspius)
  • Gynogenesis
  • Gamma Irradiation
  • Survival
  • SDY gene
  • Sex determination
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