نوع مقاله : مقاله پژوهشی
نویسندگان
مرکز تحقیقات هستهای، سازمان انرژی اتمی ایران، صندوق پستی: 8486 – 11365، تهران - ایران
چکیده
تعیین عناصر کم مقدار(2) در مایعات زیستشناختی و شناسایی بیماریها و کنترل آنها به لحاظ تشخیص سلامتی حائز اهمیّت بسیار است. افزایش غلظت این عناصر در خون ناهنجاریهایی به وجود می آورد که نتیجه آن اختلال در کار آنزیمها و سوخت و ساز (متابولیسم) بدن است. دو عنصر آرسنیک و کروم در خون معمولاً با روش فعّالسازی نوترونی(3) اندازهگیری میشوند. با توجّه به تقاضای روزافزن مراکز متعدّد درمانی و دانشگاهی برای تعیین عناصر کم مقدار، استفاده از روش طیف سنجی جذب اتمی مجهّز به کوره گرافیتی (الکتروترمال) مورد توجّه بیشتری قرار گرفته است، زیرا این روش از سهولت و حساسیت و سرعت عمل کافی برخوردار بوده و به لحاظ اقتصادی مقرون به صرفه است. در این کار پژوهشی روشهای مختلف نمونه سازی خون وسرم به منظور اندازهگیری عناصر سمّی کم مقدار آرسنیک و کروم بررسی شده و شرایط مناسب برای اندازهگیری این عناصر به وسیلة دستگاه طیف سنجی جذب اتمی مجهّز به کوره گرافیتی تعیین گردیدهاند.
کلیدواژهها
عنوان مقاله [English]
Optimization of Trace Elements Determination (Arsenic and Chromium) in Blood and Serum of Human by Electrothermal Atomic Absorption Spectrometry
نویسندگان [English]
- M.A Ahmadi Faghih
- F Aflaki
چکیده [English]
Trace elements play an important role in the biophysiology of cells by affecting their growth and contributions to various biological processes such as wound healing. Determination of toxic trace elements in biological fluids is an important subject of interest for toxicological purposes. Increasing the concentration of these elements in the blood levels, cause serious diseases in patients. Recently instrumental analysis procedures such as atomic absorption spectrometry have been used in clinical measurements for determination of many toxic trace elements in the biological samples. In this paper we are reporting the study of various methods of blood and serum samples preparation for determining the toxic trace elements of Arsenic and Chromium. The measurement of this elements performed by using electrothermal atomic absorption spectrometry. The best and reliable results for Chromium analysis was achived by injection of diluted serum samples, where the samples were diluted with HCl 0.1N. In Arsenic analysis, the best results obtained by extraction of blood with aqueous solution of TCA. For determining all of these elements the RSD% was less than 5%.
کلیدواژهها [English]
- trace elements
- atomic absorption spectrometry
- electrothermal
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