Journal of Nuclear Science, Engineering and Technology (JONSAT)
In cooperation with the Iranian Nuclear Society

Chronic exposure effect study on Ramsar high background natural radiation areas (HBNRAs) residents using micronucleus assay

Document Type : Research Paper

Authors

F. Abbasi Siar 1
P. Abdolmaleki 2
A. Haeri 1
M. Hosseini Pooya 3

1 Reactor and Nuclear Safety Research School, Nuclear Science and Technology Research Institute, AEOI, P.O.Box: 14395-836, Tehran - Iran

2 Biophysics Department, Faculty of Biological Sciences, Tarbiat Modares University, P.O.BOX: 14115-154, Tehran - Iran

3 Radiation Application Research School, Nuclear Science and Technology Research Institute, P.O.Box: 14395-836, Tehran - Iran

https://doi.org/10.24200/nst.2023.1345
Abstract
The effect of chronic exposure on Ramsar HBNRAs inhabitants' health was studied using Cytokinesis Blocked Micronucleus (CBMN) Assay. The results showed a significant difference (P <0.001) in the mean frequency of micronucleus of the study group, or resident in Ramsar HBNRAs, and the control group, which were selected from Tonekabon, a city near Ramsar. Significant differences (P <0.001) were observed between the mean micronucleus frequency after irradiation of blood samples, which means that after irradiation of blood samples, the control group showed a higher increase in micronuclei frequency than the study group that can be as a result of the existence of a radio adaptive response in Ramsar HBNRAs. The correlation was observed between the frequency of micronucleus and age in control groups but was not obtained for the study group.

Highlights

  1. A. Shabestani Monfared, H. Mozdarani, Is it time to shed some light on the black box of health policies regarding the inhabitants of the high background radiation areas of Ramsar?, Iran. J. Radiat. Res., 10(3-4), 111-116 (2012).

 

  1. M. Sohrabi M, S.A. Durrani, In high levels of natural radiation, eds. International Atomic Energy Authority, Vienna, Austria, (1990).

 

  1. UNSCEAR (United Nations Scientific Committee on the Effects of Atomic Radiation Sources and effects of ionizing radiation), United Nations. New York, (2000).

 

  1. M. Sohrabi, Recent radiological studies of high level natural radiation areas of Ramsar, Proceeding of International Conference of High Levels of Natural Radiation (ICHLNR). Ramsar-Iran, PP: 3-7, (1990).

 

  1. S.M.J. Mortazavi, M. Ghiassi-nejad, T. Ikushima, Do the findings on the health effects of prolonged exposure to very high levels of natural radiation contradict ultra-conservative radiation protection regulations, In: Radiation and Homeostasis. Eds, Elsevier. Amsterdam, 35-37 (2002).

 

  1. A. Shabestani Monfared, et al, Association between Local External Gamma Rays and Frequency of Cancer in Babol-Iran, Dose Response, 8(3), 368-77 (2009).

 

  1. A. Shabestani Monfared, et al, The inhabitants health status in high and low natural background radiation areas in Ramsar (North of Iran), Journal of Gorganuniversity of Medhcal Sciences, 6(13), 23-29, (2004).

 

  1. S.M.J. Mortazavi, et al, Cancer Incidence in Areas with Elevated Levels of Natural Radiation, Int J Low Radiation, 2(1/2), 20-27 (2006).

 

  1. A. Shabestani Monfared, et al, High natural background radiation areas in Ramsar, Iran: can inhabitants feel safe? Int J Low Radiation, 3(2/3), 171-177 (2006).

 

10 F. Zakeri, et al., Chromosome aberrations in peripheral blood lymphocytes of individuals living in high background radiation areas of Ramsar, Iran Radiat Environ Biophys, 50, 571‐8 (2011).

 

  1. S.M.J. Mortazavi, H. Mozdarani, Non-linear phenomena in biological findings of the residents of high background radiation areas of Ramsar, Int. J. Radiat. Res., 11(1), 4-9 (2013).

 

  1. S. Borzooei Sileh, A. Shabestani Monfared, Natural Exposure, Radiadaptive response and radiation hormesis, Journal of Babol Medical Sciences, 17(1), 15-21 (2014) (In Pesian).

 

  1. IAEA. Cytogenetic Analysis for Radiation Dose Assessment, A Manual Technical reports series no. 405, Vienna, (2001).

 

  1. IAEA. Cytogenetic Dosimetry: Applications in Preparedness for and Responseto Radiation Emergencies, IAEA-EPR, Vienna, (2010).

 

  1. IRAN National Standard No. 1662, Radiation Protection, Criteria for biological dosimetry laboratories using cytogenetic methods, (2006) (In Persian).

 

  1. IRAN National Standard No. 1662, Radiation Protection, Criteria for cytogenetic triage laboraories, (2009), (In Persian).

 

  1. A. Vral, M. Fenech, H. Thierens, The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure, Mutagenesis, 26(1), 11–17, (2011).

 

  1. E.N. Ramachandran, et al, Radio-adaptive response in peripheral blood lymphocytes of individuals residing in high-level natural radiation areas of Kerala in the southwest coast of India, Mutagenesis, 00, 1–7 (2016).

 

  1. P. Atanasova, et al, The micronucleui frequency as a biological dosimeter of absorbed dose in case of radiation accident, Trakia Journal of Sciences, 7(4), 28-32 (2009).

 

  1. M.E. Mendes, et al, Dose-response calibration curve for micronucleus assay: preliminary, X Regional Latin American Congress IRPA of Protection and Radiological Safety "Radioprotection: New Challenges for a World in Evolution", Buenos Aires, April 12 to 17, Argentine Radio Protection Company, (2015).

 

  1. E. Rastkhah, et al, The cytokinesis-blocked micronucleus assay: dose-response calibration curve, background frequency in the population and dose estimation, Radiat Environ Biophys, 55(1), 41-51 (2016).

 

  1. P. Atanasova, et al, The micronucleui frequency as a biological dosimeter of absorbed dose in case of radiation accident, Trakia Journal of Sciences, 7(4), 28-32 (2009).

 

  1. T. Allahverdi Pourfallah, H. Babapour, M. Shahidi, Evaluation of High Level Environmental Background Radiation Areas and its Variation in Ramsar, Iranian Journal of Medical Physics, 9(2), 87-92 (2012).

 

  1. M. Amirzadi, et al, Complementary measurements of Radon concentration in water sources and natural exposure in dwellings in the vicinity of the Ramsar HLNRA, Iran. Nuclear Technology & Radiation Protection, 27(4), 399-403 (2012).

 

  1. M. Syaifudin, et al, Micronucleus Assay‑based Evaluation of Radiosensitivity of Lymphocytes among Inhabitants Living in High Background Radiation Area of Mamuju, West Sulawesi, Indonesia, Genome Integrity, 2, 9(2), 1-5 (2018).

 

  1. SH. Mohammadi, et al, Adaptive Response of Blood Lymphocytes of Inhabitants Residing in High background Radiation Areas of Ramsar- Micronuclei, Apoptosis and Comet Assays, J. Radiat. Res., 47, 279-285 (2006).

 

  1. C. Bolognesi, et al, Chromosomal damage and ageing: Effect on micronuclei frequency in peripheral blood lymphocytes, Age & Ageing, 28, 393-397 (1999).

Keywords

High natural radiation
Biological dosimetry
Micro nucleus (MN) assay
Chronic exposure
Radioadaptive response
  1. A. Shabestani Monfared, H. Mozdarani, Is it time to shed some light on the black box of health policies regarding the inhabitants of the high background radiation areas of Ramsar?, Iran. J. Radiat. Res., 10(3-4), 111-116 (2012).

 

  1. M. Sohrabi M, S.A. Durrani, In high levels of natural radiation, eds. International Atomic Energy Authority, Vienna, Austria, (1990).

 

  1. UNSCEAR (United Nations Scientific Committee on the Effects of Atomic Radiation Sources and effects of ionizing radiation), United Nations. New York, (2000).

 

  1. M. Sohrabi, Recent radiological studies of high level natural radiation areas of Ramsar, Proceeding of International Conference of High Levels of Natural Radiation (ICHLNR). Ramsar-Iran, PP: 3-7, (1990).

 

  1. S.M.J. Mortazavi, M. Ghiassi-nejad, T. Ikushima, Do the findings on the health effects of prolonged exposure to very high levels of natural radiation contradict ultra-conservative radiation protection regulations, In: Radiation and Homeostasis. Eds, Elsevier. Amsterdam, 35-37 (2002).

 

  1. A. Shabestani Monfared, et al, Association between Local External Gamma Rays and Frequency of Cancer in Babol-Iran, Dose Response, 8(3), 368-77 (2009).

 

  1. A. Shabestani Monfared, et al, The inhabitants health status in high and low natural background radiation areas in Ramsar (North of Iran), Journal of Gorganuniversity of Medhcal Sciences, 6(13), 23-29, (2004).

 

  1. S.M.J. Mortazavi, et al, Cancer Incidence in Areas with Elevated Levels of Natural Radiation, Int J Low Radiation, 2(1/2), 20-27 (2006).

 

  1. A. Shabestani Monfared, et al, High natural background radiation areas in Ramsar, Iran: can inhabitants feel safe? Int J Low Radiation, 3(2/3), 171-177 (2006).

 

10 F. Zakeri, et al., Chromosome aberrations in peripheral blood lymphocytes of individuals living in high background radiation areas of Ramsar, Iran Radiat Environ Biophys, 50, 571‐8 (2011).

 

  1. S.M.J. Mortazavi, H. Mozdarani, Non-linear phenomena in biological findings of the residents of high background radiation areas of Ramsar, Int. J. Radiat. Res., 11(1), 4-9 (2013).

 

  1. S. Borzooei Sileh, A. Shabestani Monfared, Natural Exposure, Radiadaptive response and radiation hormesis, Journal of Babol Medical Sciences, 17(1), 15-21 (2014) (In Pesian).

 

  1. IAEA. Cytogenetic Analysis for Radiation Dose Assessment, A Manual Technical reports series no. 405, Vienna, (2001).

 

  1. IAEA. Cytogenetic Dosimetry: Applications in Preparedness for and Responseto Radiation Emergencies, IAEA-EPR, Vienna, (2010).

 

  1. IRAN National Standard No. 1662, Radiation Protection, Criteria for biological dosimetry laboratories using cytogenetic methods, (2006) (In Persian).

 

  1. IRAN National Standard No. 1662, Radiation Protection, Criteria for cytogenetic triage laboraories, (2009), (In Persian).

 

  1. A. Vral, M. Fenech, H. Thierens, The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure, Mutagenesis, 26(1), 11–17, (2011).

 

  1. E.N. Ramachandran, et al, Radio-adaptive response in peripheral blood lymphocytes of individuals residing in high-level natural radiation areas of Kerala in the southwest coast of India, Mutagenesis, 00, 1–7 (2016).

 

  1. P. Atanasova, et al, The micronucleui frequency as a biological dosimeter of absorbed dose in case of radiation accident, Trakia Journal of Sciences, 7(4), 28-32 (2009).

 

  1. M.E. Mendes, et al, Dose-response calibration curve for micronucleus assay: preliminary, X Regional Latin American Congress IRPA of Protection and Radiological Safety "Radioprotection: New Challenges for a World in Evolution", Buenos Aires, April 12 to 17, Argentine Radio Protection Company, (2015).

 

  1. E. Rastkhah, et al, The cytokinesis-blocked micronucleus assay: dose-response calibration curve, background frequency in the population and dose estimation, Radiat Environ Biophys, 55(1), 41-51 (2016).

 

  1. P. Atanasova, et al, The micronucleui frequency as a biological dosimeter of absorbed dose in case of radiation accident, Trakia Journal of Sciences, 7(4), 28-32 (2009).

 

  1. T. Allahverdi Pourfallah, H. Babapour, M. Shahidi, Evaluation of High Level Environmental Background Radiation Areas and its Variation in Ramsar, Iranian Journal of Medical Physics, 9(2), 87-92 (2012).

 

  1. M. Amirzadi, et al, Complementary measurements of Radon concentration in water sources and natural exposure in dwellings in the vicinity of the Ramsar HLNRA, Iran. Nuclear Technology & Radiation Protection, 27(4), 399-403 (2012).

 

  1. M. Syaifudin, et al, Micronucleus Assay‑based Evaluation of Radiosensitivity of Lymphocytes among Inhabitants Living in High Background Radiation Area of Mamuju, West Sulawesi, Indonesia, Genome Integrity, 2, 9(2), 1-5 (2018).

 

  1. SH. Mohammadi, et al, Adaptive Response of Blood Lymphocytes of Inhabitants Residing in High background Radiation Areas of Ramsar- Micronuclei, Apoptosis and Comet Assays, J. Radiat. Res., 47, 279-285 (2006).

 

  1. C. Bolognesi, et al, Chromosomal damage and ageing: Effect on micronuclei frequency in peripheral blood lymphocytes, Age & Ageing, 28, 393-397 (1999).

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