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Evaluation of the effect of deuterium-depleted water on inhibiting the growth of colorectal cancer cells

Document Type : Research Paper

Authors

1 Department of Food Sciences and Technology, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, P.O.Box: 14515-775, Tehran - Iran

2 Nuclear Fuel Cycle Research School, Nuclear Science and Technology Research Institute, AEOI, P.O.Box:11365-8486, Tehran-Iran

Abstract

This study aimed to investigate the effect of deuterium-depleted water (DDW) in specific concentrations on HT-29 and SW-480 cells. HT-29 and SW-480 cell lines were cultured in RPMI medium containing different concentrations of DDW for 24, 48, and 72 hours, and then the percentage of cell survival was determined by MTT-based cytotoxicity assay. The findings proved the role of reducing deuterium and increasing time treatment in inhibiting the growth of these cell lines. The lowest cell survival rates for HT-29 and SW-480 cell lines were obtained during 72 hours of treatment with 30 ppm deuterium concentration, with 23% and 37% cell survival percentages, respectively. To investigate the side effects of deuterium-depleted water, normal C2C12 cells were also treated with deuterium-depleted water. The results showed that under the same conditions as cancer cells, deuterium-depleted water has a lower growth inhibitory effect on normal cells, so cell survival in this cell line was more than 50%. Our data show that deuterium-depleted water could open up a new strategic approach to preventing and treating colorectal cancer.

Highlights

  1. C.M. Villanueva, et al, Exposure to widespread drinking water chemical, blood inflammation markers and colorectal cancer, Environment International, 10, 68-73(2021).

 

  1. N.K. Sharma, et al, Dietary Choices Modulate Colorectal Cancer Stem Cells: A Role of FXR Nuclear Receptor, Nutrition and Cancer, 73(7), 1-8 (2020).

 

  1. J. Yin, et al, Identification of an at-risk sub population with high immune infiltration based on the peroxispme pathway and TIM3 in colorectal cancer, BMC Cancer, 22, 44 (2022).

 

  1. D. Wu, et al, Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro, Cell Cycle, 17(13), 1579-1590 (2020).

 

  1. D.M. Muzny, et al, Comprehensive molecular characterization of human colon and rectal cancer, Journal of Nature, 487, 330-337 (2012).

 

  1. J. Wharam, et al, Colorectal cancer screening in a nationwide high-deductible health plan before and after the Affordable Care Act, Med Care, 54, 466–473 (2016).

 

  1. E.G. Luebeck, S.H. Moolgavkar, Multistage carcinogenesis and the incidence of colorectal cancer, Proc Natl AcadSci USA, 99(23), 15095-100 (2002).

 

  1. M.G. Priebe, et al, The physiology of colonic metabolism possibilities for interventions with pre- and probiotics, Eur J Nutr, 41(12), 10 (2002).

 

  1. H. Krueger, D. McLean, D. Williams, Gastrointestinal cancers, Prog Exp Tumor Res, 40, 85-91 (2008).

 

  1. D. Sur D, et al, Treatment of colorectal cancer: actual strategies and promising perspectives, World Journal of Pharmacy and Pharmaceutical Sciences, 7(8), 32-44 (2018).

 

  1. G.A. Chung-Faye, D.J. Kerr, Innovative treatment for colon cancer, BMJ, 321, 1397-9 (2000).

 

  1. R. Xu, et al, Recent advances in the treatment of colon cancer, HistolHistopathol, 21, 867-72 (2006).

 

  1. C. Meladin, et al, Deuterium-depleted water has stimulating effects on long - termmemory in rats, Journal of Neuroscience Letters, 7:583,154-8 (2014).

 

  1. G. Somlyai, et al, Effect of systemic subnormal deuterium level on metabolic syndrome related and other blood parameters in humans: a preliminary study, Molecules, 25(6), 1376 (2020).

 

  1. F.S. Cong, et al, Deuterium-depleted water inhibits human lung carcinoma cell growth by apoptosis, Exp Ther Med, 1, 277-283 (2010).

 

  1. Z. Gyongyi, et al, Deuterium depleted water effects on survival of lung cancer patients and expression of Kras, Bcl2, and Myc genes in mouse lung, Nutr Cancer, 65, 240–246 (2013).

 

  1. A. Kovacs, et al, Deuterium Depletion May Delay the Progression of Prostate Cancer, Journal of Cancer Therapy, 2(4), (2011).

 

  1. S. Soleyman-Jahi, et al, In vitro assessment of antineoplastic effects of deuterium depleted water, Asian Pac J Cancer Prev, 15, 2179-2183 (2014).

 

  1. H. Wang, et al, Deuterium-depleted water (DDW) selectively inhibits the proliferation and migration of nasopharyngeal carcinoma cell in vitro, Biomed Pharmacother, 67(6), 489-96 (2013).

 

  1. A. Syroeshkin, Deuterium depleted water as an adjuvant in treatment of cancer, Sys Rev Pharm, 10(1), 112-117 (2019).

Keywords

References
  1. C.M. Villanueva, et al, Exposure to widespread drinking water chemical, blood inflammation markers and colorectal cancer, Environment International, 10, 68-73(2021).

 

  1. N.K. Sharma, et al, Dietary Choices Modulate Colorectal Cancer Stem Cells: A Role of FXR Nuclear Receptor, Nutrition and Cancer, 73(7), 1-8 (2020).

 

  1. J. Yin, et al, Identification of an at-risk sub population with high immune infiltration based on the peroxispme pathway and TIM3 in colorectal cancer, BMC Cancer, 22, 44 (2022).

 

  1. D. Wu, et al, Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro, Cell Cycle, 17(13), 1579-1590 (2020).

 

  1. D.M. Muzny, et al, Comprehensive molecular characterization of human colon and rectal cancer, Journal of Nature, 487, 330-337 (2012).

 

  1. J. Wharam, et al, Colorectal cancer screening in a nationwide high-deductible health plan before and after the Affordable Care Act, Med Care, 54, 466–473 (2016).

 

  1. E.G. Luebeck, S.H. Moolgavkar, Multistage carcinogenesis and the incidence of colorectal cancer, Proc Natl AcadSci USA, 99(23), 15095-100 (2002).

 

  1. M.G. Priebe, et al, The physiology of colonic metabolism possibilities for interventions with pre- and probiotics, Eur J Nutr, 41(12), 10 (2002).

 

  1. H. Krueger, D. McLean, D. Williams, Gastrointestinal cancers, Prog Exp Tumor Res, 40, 85-91 (2008).

 

  1. D. Sur D, et al, Treatment of colorectal cancer: actual strategies and promising perspectives, World Journal of Pharmacy and Pharmaceutical Sciences, 7(8), 32-44 (2018).

 

  1. G.A. Chung-Faye, D.J. Kerr, Innovative treatment for colon cancer, BMJ, 321, 1397-9 (2000).

 

  1. R. Xu, et al, Recent advances in the treatment of colon cancer, HistolHistopathol, 21, 867-72 (2006).

 

  1. C. Meladin, et al, Deuterium-depleted water has stimulating effects on long - termmemory in rats, Journal of Neuroscience Letters, 7:583,154-8 (2014).

 

  1. G. Somlyai, et al, Effect of systemic subnormal deuterium level on metabolic syndrome related and other blood parameters in humans: a preliminary study, Molecules, 25(6), 1376 (2020).

 

  1. F.S. Cong, et al, Deuterium-depleted water inhibits human lung carcinoma cell growth by apoptosis, Exp Ther Med, 1, 277-283 (2010).

 

  1. Z. Gyongyi, et al, Deuterium depleted water effects on survival of lung cancer patients and expression of Kras, Bcl2, and Myc genes in mouse lung, Nutr Cancer, 65, 240–246 (2013).

 

  1. A. Kovacs, et al, Deuterium Depletion May Delay the Progression of Prostate Cancer, Journal of Cancer Therapy, 2(4), (2011).

 

  1. S. Soleyman-Jahi, et al, In vitro assessment of antineoplastic effects of deuterium depleted water, Asian Pac J Cancer Prev, 15, 2179-2183 (2014).

 

  1. H. Wang, et al, Deuterium-depleted water (DDW) selectively inhibits the proliferation and migration of nasopharyngeal carcinoma cell in vitro, Biomed Pharmacother, 67(6), 489-96 (2013).

 

  1. A. Syroeshkin, Deuterium depleted water as an adjuvant in treatment of cancer, Sys Rev Pharm, 10(1), 112-117 (2019).
Journal of Nuclear Science, Engineering and Technology (JONSAT)
Volume 44, Issue 1 - Serial Number 103
April 2023
Pages 97-102
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