Journal of Nuclear Science, Engineering and Technology (JONSAT)
In cooperation with the Iranian Nuclear Society

Preparation, quality control, biodistribution of [113mIn]In-DOTATATE

Document Type : Research Paper

Authors

F. Bolourinovin 1, 2
M. Mirzaei 1
R. Faghihi 2
F. Joharidaha 1
S. Sina 2
K. Hadad 2
F. Badipa 1
H. Yousefnia 1

1 Radiation Application Research School, Nuclear Science and Technology Research Institute, AEOI, P. O. Box 11365-3486, Tehran - Iran

2 Department of Nuclear Engineering, Faculty of Mechanical Engineering, Shiraz University, P.O.Box: 7193616548, Shiraz - Iran

https://doi.org/10.24200/nst.2024.1526
Abstract
In this study, to produce and develop a new diagnostic radiopharmaceutical for the imaging of tumors with overexpressed somatostatin receptor, the [113mIn]In-DOTATATE chemical compound was prepared in optimal conditions using an in-house generator, and its preclinical studies were performed. The final product was obtained with a radiochemical purity of nearly 99% (RTLC and HPLC) and a specific activity of 26.2×1015 Bq/mol. The investigation of the stability of the labeled compound showed a radiochemical purity of more than 96% for at least three hours in PBS buffer and human blood serum. The results of the biodistribution and image assessment of the [113mIn]In-DOTATATE chemical compound in normal mice showed that the organs of the gastrointestinal tract such as the pancreas, stomach, and intestine, which have cells expressing the somatostatin receptor, have high uptake.

Highlights

  1. Singh H. Helping health care organizations to define diagnostic errors as missed opportunities in diagnosis Joint Commission. J Qual Patient Safety. 2014;40: 99–101.

 

  1. Knapp F.F, Mirzadeh S. The continuing important role of radionuclide generator systems for nuclear medicine. Eur. J. Nucl. Med. 1994;21:1151-1165.

 

  1. Ruth T.J. Annual Review of Nuclear and Particle Science The Shortage of Technetium-99m and Possible Solutions. Annu. Rev. Nucl. Part. Sci. 2020;70:77-94.

 

  1. Chu S.Y.F. The Lund/LBNL Nuclear Data Search. Version 2.0, February. http://nucleardata.nuclear.lu.se/nucleardata/toi/index.asp (1999).

 

  1. Stern H.S, Zolle I, McAfee J.G. Preparation of technetium (Tc99m)-labeled serum albumin (human). Int. J. Appl. Radiat. Isot. 1965;16:283-288.

 

  1. Brookeman V.A, Sun P.C.J, Bruno F.P, Dunavant B.G, Mauderli W. Internal distribution and absorbed dose calculations for radioactive indium liver and lung scanning agents. Am. J. Roentgenol. 1970;109.

 

  1. O'Mara R.E, Subramanian G, McAfee J.G, Burger C.L. Comparison of 113mIn and other short lived agents for cerebral scanning. J. Nucl. Med. 1969;10:18-27.

 

  1. Mostafa M, El-Sadek A.A, El-Said H, El-Amir M.A. 99Mo/99mTc–113Sn/113mIn Dual Radioisotope Generator Based on 6-Tungstocerate(IV) Column Matrix. J. Nucl. Radiochem. Sci. 2009;10:1-12.

 

  1. Schirmer W.J, Melvin W.S, Rush R.M, O'Dorisio Th.M, Pozderac R.V, Olsen J.O, Ellison E.C. Indium-111-pentetreotide scanning versus conventional imaging techniques for the localization of gastrinoma. Surgery. 1995;118:1105–1113.

 

  1. Aghanejad A, Jalilian A.R, Maus S, Yousefnia H, Geramifar P, Beiki D. Optimized production and quality control of 68Ga-DOTATATE. Iran J Nucl Med. 2016;24(1):29-3.

 

  1. Raj N, Reidy-Lagunes D. The Role of 68Ga-DOTATATE PET/CT in Well Differentiated Neuroendocrine Tumors: A Case-Based Approach Illustrates Potential Benefits and Challenges. Pancreas. 2018;47:1–5.

 

  1. Velikyan I, Sundin A, Sörensen J, Lubberink M, Sandström M, Garske-Román U, Lundqvist H, Granberg D, Eriksson B. Quantitative and qualitative intrapatient comparison of 68Ga-DOTATOC and 68GaDOTATATE: net uptake rate for accurate quantification. J. Nucl. Med. 2014;55:204-210.

 

  1. Yousefnia H, Zolghadri S, Jalilian A.R, Tajik M, Ghannadi-Maragheh M. Preliminary dosimetric evaluation of 166Ho-TTHMP for human based on biodistribution data in rats. Appl. Rad. Iso. 2014;94:260-265.

Keywords

Indium-113m
DOTATATE
SPECT
Biodistribution
  1. Singh H. Helping health care organizations to define diagnostic errors as missed opportunities in diagnosis Joint Commission. J Qual Patient Safety. 2014;40: 99–101.

 

  1. Knapp F.F, Mirzadeh S. The continuing important role of radionuclide generator systems for nuclear medicine. Eur. J. Nucl. Med. 1994;21:1151-1165.

 

  1. Ruth T.J. Annual Review of Nuclear and Particle Science The Shortage of Technetium-99m and Possible Solutions. Annu. Rev. Nucl. Part. Sci. 2020;70:77-94.

 

  1. Chu S.Y.F. The Lund/LBNL Nuclear Data Search. Version 2.0, February. http://nucleardata.nuclear.lu.se/nucleardata/toi/index.asp (1999).

 

  1. Stern H.S, Zolle I, McAfee J.G. Preparation of technetium (Tc99m)-labeled serum albumin (human). Int. J. Appl. Radiat. Isot. 1965;16:283-288.

 

  1. Brookeman V.A, Sun P.C.J, Bruno F.P, Dunavant B.G, Mauderli W. Internal distribution and absorbed dose calculations for radioactive indium liver and lung scanning agents. Am. J. Roentgenol. 1970;109.

 

  1. O'Mara R.E, Subramanian G, McAfee J.G, Burger C.L. Comparison of 113mIn and other short lived agents for cerebral scanning. J. Nucl. Med. 1969;10:18-27.

 

  1. Mostafa M, El-Sadek A.A, El-Said H, El-Amir M.A. 99Mo/99mTc–113Sn/113mIn Dual Radioisotope Generator Based on 6-Tungstocerate(IV) Column Matrix. J. Nucl. Radiochem. Sci. 2009;10:1-12.

 

  1. Schirmer W.J, Melvin W.S, Rush R.M, O'Dorisio Th.M, Pozderac R.V, Olsen J.O, Ellison E.C. Indium-111-pentetreotide scanning versus conventional imaging techniques for the localization of gastrinoma. Surgery. 1995;118:1105–1113.

 

  1. Aghanejad A, Jalilian A.R, Maus S, Yousefnia H, Geramifar P, Beiki D. Optimized production and quality control of 68Ga-DOTATATE. Iran J Nucl Med. 2016;24(1):29-3.

 

  1. Raj N, Reidy-Lagunes D. The Role of 68Ga-DOTATATE PET/CT in Well Differentiated Neuroendocrine Tumors: A Case-Based Approach Illustrates Potential Benefits and Challenges. Pancreas. 2018;47:1–5.

 

  1. Velikyan I, Sundin A, Sörensen J, Lubberink M, Sandström M, Garske-Román U, Lundqvist H, Granberg D, Eriksson B. Quantitative and qualitative intrapatient comparison of 68Ga-DOTATOC and 68GaDOTATATE: net uptake rate for accurate quantification. J. Nucl. Med. 2014;55:204-210.

 

  1. Yousefnia H, Zolghadri S, Jalilian A.R, Tajik M, Ghannadi-Maragheh M. Preliminary dosimetric evaluation of 166Ho-TTHMP for human based on biodistribution data in rats. Appl. Rad. Iso. 2014;94:260-265.

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