Journal of Nuclear Science, Engineering and Technology (JONSAT)
In cooperation with the Iranian Nuclear Society

Screening of Significant Factors in Uranium Biosorption from Aqueous Solutions Using Pseudomonas Putida Immobilized on Chitosan

Document Type : Research Paper

Authors

H Sohbatzadeh Lonbar
A Keshtkar
J Safdari
F Fatemi Ardestani
M Ghasemi Torkabadi
Abstract
Heavy metal sequestration from industrial wastewater is a serious environmental problem especially in developing countries. Among various treatment technologies, it seems that biosoprtion is a promising alternative method. Microbial–based biosorbents are effective and applicable for heavy metals removal from aqueous solutions. The present study investigated the ability of Pseudomonas putida immobilized on chitosan to adsorb uranium (VI) from the aqueous solution. The biosorption process factors were screened using Plackett–Burman design. The results showed that the biosorbent dosage, initial concentration, biosorbent particle size, bacteria wt.% in the biosorbent and pH were the most significant parameters, respectively while temperature was the only insignificant parameter in the biosorption process. Maximum practical biosorption capacity was 536.08 mg g–1 obtained from 15 wt.% of the bacterial cells immobilized on chitosan. Biosorption equilibrium isotherms were analyzed by Langmuir, Freundlich and Dubinin–Radushkevhch models. For Pseudomonas putida immobilized on chitosan, the Langmuir isotherm model (R2=0.983) was proved to fit the equilibrium data best with the maximum capacities of 588.23 and 454.54 mg g–1 for the biosorbent and pure chitosan, respectively. In conclusion, the present study indicated that the prepared composite biosorbent could be a suitable candidate for uranium (VI) biosorption.

Highlights

[1] M. Ahmaruzzaman, Industrial wastes as low-cost potential adsorbents for the treatment of wastewater laden with heavy metals, Adv. Colloid Interfac. 166 (2011) 36-59.

 

[2] D.A. Carvajal, Y.P. Katsenovich, L.E. Lagos, The effects of aqueous bicarbonate and calcium ions on uranium biosorption by Arthrobacter G975 strain, Chem. Geol. 330-331 (2012) 51-59.

 

[3] J. Wang, C. Chen, Biosorbents for heavy metals removal and their future, Biotechnol. Adv. 27 (2009) 195–226.

 

[4] K. Vijayaraghavan, Y.-S. Yun, Bacterial biosorbents and biosorption, Biotechnol. Adv. 26 (2008) 266-291.

 

[5] S. Madala, S.K. Nadavala, S. Vudagandla, V.M. Boddu, K. Abburi, Equilibrium, kinetics and thermodynamics of Cadmium (II) biosorption on to composite chitosan biosorbent, Arab. J. Chem. 10 (2013) S1883-S1893.

 

[6] F. Colak, N. Atarb, D. Yazıcıoglu, A. Olgun, Biosorption of lead from aqueous solutions by Bacillus strains possessing heavy-metal resistance, Chem. Eng. J. 173 (2011) 422-428.

 

[7] A.R. Keshtkar, F. Kafshgari, Application of Ca-Pretreated Brown Alga for Heavy Metals Removal from Esfahan Uranium Conversion Facility (UCF) Wastewater, J. of Nuclear Sci. and Tech. 67 (2014) 22-30 (In Persian).

 

[8] S. Sana, R. Roostaazad, S. Yaghmaei, Biosorption of Uranium (VI) from Aqueous Solution by Pretreated Aspergillus niger Using Sodium Hydroxide, IJCCE. 34(1) (2015) 65-74.

 

[9] A. Keshtkar, M.M. Montazer Rahmati, N. Khodapanah, Application of Two-Parameter and Three-Parameter Isotherm Models at Uranium Biosorption by Baker's Yeast, J. of Nuclear Sci. and Tech. 50 (2010) 1-8 (In Persian).

 

[10] M.L. Merroun, S. Selenska-Pobell, Bacterial interactions with uranium: An environmental perspective, J. Contam. Hydrol. 102 (2008) 285–295.

 

[11] D. Park, Y.-S. Yun, J.M. Park, The Past, Present, and Future Trends of Biosorption, Biotechnol. Bioproc. E. 15 (2010) 86-102.

 

[12] P. Sar, S.K. Kazy, S.F. D’Souza, Radionuclide remediation using a bacterial biosorbent, Int. Biodeter. Biodegr. 54 (2004) 193-202.

 

[13] Z.R. Yelebe, B.Z. Yelebe, R.J. Samuel, Design of fixed bed column for the removal of metal contaminations from industrial wastewater, J. Eng. Appl. Sci. 5(2) (2013) 68-77.

 

[14] S.C.S. Martins, C.M. Martins, L.M.C.G. Fiúza, S.T. Santaella, Immobilization of microbial cells: A promising tool for treatment of toxic pollutants in industrial wastewater, Afr. J. Biotechnol. 12(28) (2013) 4412-4418.

 

[15] G. Xiao, X. Zhang, H. Su, T. Tan, Plate column biosorption of Cu(II) on membrane-type biosorbent (MBS) of Penicillium biomass: Optimization using statistical design methods, Bioresource Technol. 143 (2013) 490-498.

 

[16] J. Wang, C. Chen, Chitosan-based biosorbents: Modification and application for biosorption of heavy metals and radionuclides, Bioresource Technol. 160 (2014) 129-141.

 

[17] J. Choi, J.Y. Lee, J.-S. Yang, Biosorption of heavy metals and uranium by starfish and Pseudomonas putida, J. Hazard. Mater. 161 (2009) 157-162.

 

[18] W.S. Wan Ngah, M.A.K.M. Hanafiah, S.S. Yong, Adsorption of humic acid from aqueous solutions on crosslinked chitosan–epichlorohydrin beads: Kinetics and isotherm studies, Colloid. Surfaces B 65 (2008) 18-24.

 

[19] N.T. Abdel-Ghani, G.A. El-Chaghaby, Biosorption for metal ions removal from aqueous solutions: a review of recent studies, IJLRST. 3(1) (2014) 24-42.

 

[20] W.S. Wan Ngah, S. Fatinathan, Adsorption characterization of Pb(II) and Cu(II) ions onto chitosan-tripolyphosphate beads: Kinetic, equilibrium and thermodynamic studies, J. Environ. Manage. 91 (2010) 958-969.

 

[21] G.H. Mirzabe, A.R. Keshtkar, Application of response surface methodology for thorium adsorption on PVA/Fe3O4/SiO2/APTES nanohybrid adsorbent, J. Ind. Eng. Chem. 26 (2015) 277-285.

 

[22] Y.-g. LIU, T. LIAO, Z.-b. HE, T.-t. LI, H. WANG, X.-j. HU, Y.-m. GUO, Y. HE, Biosorption of copper(II) from aqueous solution by Bacillus subtilis cells immobilized into chitosan beads, T. Nonferr. Metal. Soc. 23 (2013) 1804-1814.

 

[23] M. Roosta, M. Ghaedi, A. Daneshfar, R. Sahraei, Experimental design based response surface methodology optimization of ultrasonic assisted adsorption of safaranin O by tin sulfide nanoparticle loaded on activated carbon, Spectrochim. Acta A 122 (2014) 223–231.

[24] P. Kotrba, M. Mackova, T. Macek, Microbial Biosorption of Metals, Springer Science+Business Media B.V., 2011.

 

[25] D. Humelnicu, M.V. Dinu, E.S. Dragan, Adsorption characteristics of UO2 2+ and Th4+ ions from simulated radioactive solutions onto chitosan/clinoptilolite sorbents, J. Hazard. Mater. 185 (2011) 447-455.

 

[26] F. Wang, L. Tan, Q. Liu, R. Li, Z. Li, H. Zhang, S. Hu, L. Liu, J. Wang, Biosorption characteristics of Uranium (VI) from aqueous solution by pollen pini, J. Environ. Radioactiv. 150 (2015) 93-98.

 

[27] J.-s. Wang, X.-j. Hu, J. Wang, Z.-l. Bao, S.-b. Xie, J.-h. Yang, The tolerance of Rhizopus arrihizus to U(VI) and biosorption behavior of U(VI) onto R. arrihizus, Biochem. Eng. J. 51 (2010) 19–23.

 

[28] K. Akhtar, M.W. Akhtar, A.M. Khalid, Removal and recovery of uranium from aqueous solutions by Trichoderma harzianum, Water Res. 41 (2007) 1366-1378.

[29] R.B. Sashidhar, S. Kalaignana Selvi, V.T.P. Vinod, T. Kosuri, D. Raju, R. Karuna, Bioprospecting of gum kondagogu (Cochlospermum gossypium) for bioremediation of uranium (VI) from aqueous solution and synthetic nuclear power reactor effluents, J. Environ. Radioactiv. 148 (2015) 33-41.

 

[30] C. Pang, Y.-H. Liu, X.-H. Cao, M. Li, G.-L. Huang, R. Hua, C.-X. Wang, Y.-T. Liu, X.-F. An, Biosorption of uranium(VI) from aqueous solution by dead fungal biomass of Penicillium citrinum, Chem. Eng. J. 170 (2011) 1-6.

 

[31] A.R. Keshtkar, M. Mohammadi, M.A. Moosavian, Equilibrium biosorption studies of wastewater U(VI), Cu(II) and Ni(II) by the brown alga Cystoseira indica in single, binary and ternary metal systems, J. Radioanal. Nucl. Ch. 303 (2015) 363–376.

Keywords

Biosorption
Uranium(VI)
Bacterium
Pseudomonas Putida
Chitosan
[1] M. Ahmaruzzaman, Industrial wastes as low-cost potential adsorbents for the treatment of wastewater laden with heavy metals, Adv. Colloid Interfac. 166 (2011) 36-59.
 
[2] D.A. Carvajal, Y.P. Katsenovich, L.E. Lagos, The effects of aqueous bicarbonate and calcium ions on uranium biosorption by Arthrobacter G975 strain, Chem. Geol. 330-331 (2012) 51-59.
 
[3] J. Wang, C. Chen, Biosorbents for heavy metals removal and their future, Biotechnol. Adv. 27 (2009) 195–226.
 
[4] K. Vijayaraghavan, Y.-S. Yun, Bacterial biosorbents and biosorption, Biotechnol. Adv. 26 (2008) 266-291.
 
[5] S. Madala, S.K. Nadavala, S. Vudagandla, V.M. Boddu, K. Abburi, Equilibrium, kinetics and thermodynamics of Cadmium (II) biosorption on to composite chitosan biosorbent, Arab. J. Chem. 10 (2013) S1883-S1893.
 
[6] F. Colak, N. Atarb, D. Yazıcıoglu, A. Olgun, Biosorption of lead from aqueous solutions by Bacillus strains possessing heavy-metal resistance, Chem. Eng. J. 173 (2011) 422-428.
 
[7] A.R. Keshtkar, F. Kafshgari, Application of Ca-Pretreated Brown Alga for Heavy Metals Removal from Esfahan Uranium Conversion Facility (UCF) Wastewater, J. of Nuclear Sci. and Tech. 67 (2014) 22-30 (In Persian).
 
[8] S. Sana, R. Roostaazad, S. Yaghmaei, Biosorption of Uranium (VI) from Aqueous Solution by Pretreated Aspergillus niger Using Sodium Hydroxide, IJCCE. 34(1) (2015) 65-74.
 
[9] A. Keshtkar, M.M. Montazer Rahmati, N. Khodapanah, Application of Two-Parameter and Three-Parameter Isotherm Models at Uranium Biosorption by Baker's Yeast, J. of Nuclear Sci. and Tech. 50 (2010) 1-8 (In Persian).
 
[10] M.L. Merroun, S. Selenska-Pobell, Bacterial interactions with uranium: An environmental perspective, J. Contam. Hydrol. 102 (2008) 285–295.
 
[11] D. Park, Y.-S. Yun, J.M. Park, The Past, Present, and Future Trends of Biosorption, Biotechnol. Bioproc. E. 15 (2010) 86-102.
 
[12] P. Sar, S.K. Kazy, S.F. D’Souza, Radionuclide remediation using a bacterial biosorbent, Int. Biodeter. Biodegr. 54 (2004) 193-202.
 
[13] Z.R. Yelebe, B.Z. Yelebe, R.J. Samuel, Design of fixed bed column for the removal of metal contaminations from industrial wastewater, J. Eng. Appl. Sci. 5(2) (2013) 68-77.
 
[14] S.C.S. Martins, C.M. Martins, L.M.C.G. Fiúza, S.T. Santaella, Immobilization of microbial cells: A promising tool for treatment of toxic pollutants in industrial wastewater, Afr. J. Biotechnol. 12(28) (2013) 4412-4418.
 
[15] G. Xiao, X. Zhang, H. Su, T. Tan, Plate column biosorption of Cu(II) on membrane-type biosorbent (MBS) of Penicillium biomass: Optimization using statistical design methods, Bioresource Technol. 143 (2013) 490-498.
 
[16] J. Wang, C. Chen, Chitosan-based biosorbents: Modification and application for biosorption of heavy metals and radionuclides, Bioresource Technol. 160 (2014) 129-141.
 
[17] J. Choi, J.Y. Lee, J.-S. Yang, Biosorption of heavy metals and uranium by starfish and Pseudomonas putida, J. Hazard. Mater. 161 (2009) 157-162.
 
[18] W.S. Wan Ngah, M.A.K.M. Hanafiah, S.S. Yong, Adsorption of humic acid from aqueous solutions on crosslinked chitosan–epichlorohydrin beads: Kinetics and isotherm studies, Colloid. Surfaces B 65 (2008) 18-24.
 
[19] N.T. Abdel-Ghani, G.A. El-Chaghaby, Biosorption for metal ions removal from aqueous solutions: a review of recent studies, IJLRST. 3(1) (2014) 24-42.
 
[20] W.S. Wan Ngah, S. Fatinathan, Adsorption characterization of Pb(II) and Cu(II) ions onto chitosan-tripolyphosphate beads: Kinetic, equilibrium and thermodynamic studies, J. Environ. Manage. 91 (2010) 958-969.
 
[21] G.H. Mirzabe, A.R. Keshtkar, Application of response surface methodology for thorium adsorption on PVA/Fe3O4/SiO2/APTES nanohybrid adsorbent, J. Ind. Eng. Chem. 26 (2015) 277-285.
 
[22] Y.-g. LIU, T. LIAO, Z.-b. HE, T.-t. LI, H. WANG, X.-j. HU, Y.-m. GUO, Y. HE, Biosorption of copper(II) from aqueous solution by Bacillus subtilis cells immobilized into chitosan beads, T. Nonferr. Metal. Soc. 23 (2013) 1804-1814.
 
[23] M. Roosta, M. Ghaedi, A. Daneshfar, R. Sahraei, Experimental design based response surface methodology optimization of ultrasonic assisted adsorption of safaranin O by tin sulfide nanoparticle loaded on activated carbon, Spectrochim. Acta A 122 (2014) 223–231.
[24] P. Kotrba, M. Mackova, T. Macek, Microbial Biosorption of Metals, Springer Science+Business Media B.V., 2011.
 
[25] D. Humelnicu, M.V. Dinu, E.S. Dragan, Adsorption characteristics of UO2 2+ and Th4+ ions from simulated radioactive solutions onto chitosan/clinoptilolite sorbents, J. Hazard. Mater. 185 (2011) 447-455.
 
[26] F. Wang, L. Tan, Q. Liu, R. Li, Z. Li, H. Zhang, S. Hu, L. Liu, J. Wang, Biosorption characteristics of Uranium (VI) from aqueous solution by pollen pini, J. Environ. Radioactiv. 150 (2015) 93-98.
 
[27] J.-s. Wang, X.-j. Hu, J. Wang, Z.-l. Bao, S.-b. Xie, J.-h. Yang, The tolerance of Rhizopus arrihizus to U(VI) and biosorption behavior of U(VI) onto R. arrihizus, Biochem. Eng. J. 51 (2010) 19–23.
 
[28] K. Akhtar, M.W. Akhtar, A.M. Khalid, Removal and recovery of uranium from aqueous solutions by Trichoderma harzianum, Water Res. 41 (2007) 1366-1378.
[29] R.B. Sashidhar, S. Kalaignana Selvi, V.T.P. Vinod, T. Kosuri, D. Raju, R. Karuna, Bioprospecting of gum kondagogu (Cochlospermum gossypium) for bioremediation of uranium (VI) from aqueous solution and synthetic nuclear power reactor effluents, J. Environ. Radioactiv. 148 (2015) 33-41.
 
[30] C. Pang, Y.-H. Liu, X.-H. Cao, M. Li, G.-L. Huang, R. Hua, C.-X. Wang, Y.-T. Liu, X.-F. An, Biosorption of uranium(VI) from aqueous solution by dead fungal biomass of Penicillium citrinum, Chem. Eng. J. 170 (2011) 1-6.
 
[31] A.R. Keshtkar, M. Mohammadi, M.A. Moosavian, Equilibrium biosorption studies of wastewater U(VI), Cu(II) and Ni(II) by the brown alga Cystoseira indica in single, binary and ternary metal systems, J. Radioanal. Nucl. Ch. 303 (2015) 363–376.

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