Journal of Nuclear Science, Engineering and Technology (JONSAT)
In cooperation with the Iranian Nuclear Society

Investigation of Antibacterial Activity of Essential Oil of Gamma Irradiated Caraway Seeds (Carum carvi L.)

Document Type : Research Paper

Authors

M Sayhoon
R Rajaie
S.L Hosseini
S Sheikh Nasiri
M Sarabi
Abstract
The antibacterial activity of essential oil extracted from gamma irradiated caraway seeds was studied by using of disk diffusion method and Minimal inhibitory concentration and Minimal bactericidal concentration. Caraway seeds were irradiated at the dosages of 0, 10 and 25 kilogray by a 60Co source of Gammacell system 220. The essential oil samples were extracted using Clevenger method and their antibacterial activities were studied by the use of disk diffusion method on four species of bacteria, namely: Escherichia coli (ATCC 25922), Pseudomonas aeroginosa (ATCC 27853), Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 29213) on the PCA and MHA culture media. Afterward, the diameters of the inhibition zones were studied. In addition, the antibacterial activity of the essential oil was compared with antibiotics such as ciprofloxacin, chloramphenicol and gentamicin on these bacteria in similar conditions. Minimal inhibitory concentration and Minimal bactericidal concentration of essential oil were also determined on these bacterial suspensions. The results showed that the antibacterial activity of the extracted essential oil from the irradiated caraway seeds. In addition, the Gram positive bacteria of Bacillus subtilis and Staphylococcus aureus were found tohave more sensitivity with respect to the Gram negative Escherichia coli and Pseudomonas aeroginosa. The results also showed that the antibacterial activity of essential oil extracted from the gamma irradiated with 25 kilogry caraway seeds decreased slightly.

Highlights

  1. 1.    I. Rasooli, L. Gachkar, D. Yadegarinia, M. B. Rezaei, M. Taghizadeh, M. H. Fakoor, A. M. Allameh, Relation of Antioxidative property and Free Radical Scavenging Capacity to the Antimicrobial Characteristics if Essential Oils from Mentha spicata L. and Chenopodium ambrosioids L. Iranian Jornal of Medical And Aromatic Plants, 23(4) (2007) 492-503.

 

  1. 2.    I. Rasooli, D. Yadegarinia, L. Gachkar, M. B. Rezaei, M. H. Fakoor, A. M. Allameh, Inhibition of Fungus Aflatoxin Production of Aspergillus parasiticus by Essential Oils, Journal of Research and Development (Watershed Management), 3(81) (2008) 146.

 

  1. 3.    C. C. C. R. De Carvalho, M. M. R. Da Fonseca, Carvone: why and how should one bother to produce this terpene, Food Chemistry, 95 (2006) 413-422.

 

  1. 4.    S. N. Mahindru, Food preservation and irradiation, 1st Ed. Saujana, New Delhi (2005) 231.

 

  1. 5.    A. Dadkhah, H. Khalafi, R. Rajaee, A. Allameh, M. Seyhoon, Study of the effects of Gamma-Irradiation on Microbial Load and Efficient Extacts of Caraway Seeds, J. of  Nuclear Sci. and Tech., 49 (2005) 27-34.

 

  1. 6.    D. Pearson, The chemical analysis of foods, 9th Ed. Churchil Luvingston, London (1976) 290-292.

 

  1. 7.    D. Yadegarinia, L. Gachkar, M. B. Rezaei, M. Taghizadeh, S. Alipoor, I. Rasooli, Biochemical activities iranian mentha piperrita L. and Myrtus commumis L. Essential Oils, Phytochemistry 67 (2006) 1249-1255.
  2. 8.    G. Singh, I. P. S. Kapoor, S. K. Pandey, U. K. Singh, R. K. Singh, Studies on Essential Oil: Part 10; Antibacterial Activity of Volatile Oil of Some Spices, Phytother. Res. 16 (2002) 680-682.

 

  1. 9.    E. J. Baron and S. M. Finegold, Bailey and Scott, Diagnostic microbiology, 8th Ed. Mosby (1990) 171-179.

 

10. NCCLS M2-A7, Performance standards for antimicrobial disk susceptibility tests; approved, Standard-Seventh Edition (2000).

 

11. S. Bouhdid, S. N. Skali, M. Idaomar, A. Zhiri, D. Baudoux, M. Amensour, J. Abrini, Antibaterial and antioxidant activities of Origanum compactum essential oil, African Journal of Biotechnology 7(10) (2008) 1563-1570.

 

12. M. Mohsenzadeh, Evaluation of antibacterial activity of selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium, Pakistan Journal of  Biological Science, 10(20) (2007) 3693-3697.

 

13. N. S. Iacobellis, P. Lo Cantore, F. Capasso, F. Senatore, Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils, Journal of Agricultural and Food Chemistry, 53(1) (2005) 57-61.

 

14. S. Burt, Essential oils: their antibacterial properties and potential applications in foods-a review, International Journal of Food Microbiology, 94(3) (2004) 223-253.

 

 

Keywords

Gamma Irradiation
Caraway
Essential Oil
Antibacterial Activity
  1. 1.    I. Rasooli, L. Gachkar, D. Yadegarinia, M. B. Rezaei, M. Taghizadeh, M. H. Fakoor, A. M. Allameh, Relation of Antioxidative property and Free Radical Scavenging Capacity to the Antimicrobial Characteristics if Essential Oils from Mentha spicata L. and Chenopodium ambrosioids L. Iranian Jornal of Medical And Aromatic Plants, 23(4) (2007) 492-503.

 

  1. 2.    I. Rasooli, D. Yadegarinia, L. Gachkar, M. B. Rezaei, M. H. Fakoor, A. M. Allameh, Inhibition of Fungus Aflatoxin Production of Aspergillus parasiticus by Essential Oils, Journal of Research and Development (Watershed Management), 3(81) (2008) 146.

 

  1. 3.    C. C. C. R. De Carvalho, M. M. R. Da Fonseca, Carvone: why and how should one bother to produce this terpene, Food Chemistry, 95 (2006) 413-422.

 

  1. 4.    S. N. Mahindru, Food preservation and irradiation, 1st Ed. Saujana, New Delhi (2005) 231.

 

  1. 5.    A. Dadkhah, H. Khalafi, R. Rajaee, A. Allameh, M. Seyhoon, Study of the effects of Gamma-Irradiation on Microbial Load and Efficient Extacts of Caraway Seeds, J. of  Nuclear Sci. and Tech., 49 (2005) 27-34.

 

  1. 6.    D. Pearson, The chemical analysis of foods, 9th Ed. Churchil Luvingston, London (1976) 290-292.

 

  1. 7.    D. Yadegarinia, L. Gachkar, M. B. Rezaei, M. Taghizadeh, S. Alipoor, I. Rasooli, Biochemical activities iranian mentha piperrita L. and Myrtus commumis L. Essential Oils, Phytochemistry 67 (2006) 1249-1255.
  2. 8.    G. Singh, I. P. S. Kapoor, S. K. Pandey, U. K. Singh, R. K. Singh, Studies on Essential Oil: Part 10; Antibacterial Activity of Volatile Oil of Some Spices, Phytother. Res. 16 (2002) 680-682.

 

  1. 9.    E. J. Baron and S. M. Finegold, Bailey and Scott, Diagnostic microbiology, 8th Ed. Mosby (1990) 171-179.

 

10. NCCLS M2-A7, Performance standards for antimicrobial disk susceptibility tests; approved, Standard-Seventh Edition (2000).

 

11. S. Bouhdid, S. N. Skali, M. Idaomar, A. Zhiri, D. Baudoux, M. Amensour, J. Abrini, Antibaterial and antioxidant activities of Origanum compactum essential oil, African Journal of Biotechnology 7(10) (2008) 1563-1570.

 

12. M. Mohsenzadeh, Evaluation of antibacterial activity of selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium, Pakistan Journal of  Biological Science, 10(20) (2007) 3693-3697.

 

13. N. S. Iacobellis, P. Lo Cantore, F. Capasso, F. Senatore, Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils, Journal of Agricultural and Food Chemistry, 53(1) (2005) 57-61.

 

14. S. Burt, Essential oils: their antibacterial properties and potential applications in foods-a review, International Journal of Food Microbiology, 94(3) (2004) 223-253.

 

 

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