Journal of Nuclear Science, Engineering and Technology (JONSAT)
In cooperation with the Iranian Nuclear Society

Evaluation of Effective Parameters on Labeling of Hydroxyapatite Compound with 90Y and Introducing the Best Method to Produce 90Y-HAp Radiopharmaceutical for Radiosynovectomy

Document Type : Research Paper

Authors

M.R Davarpanah
H.A Khoshhosn
S Attar Nosrati
S.M Harati
S.M Aghamiri
M Ghannadi Maragheh
Abstract
Radiosynovectomy is a local intra-articular injection of radionuclides in colloidal form for treatment of articular inflammatory in rheumatoid arthritis, hemophilia or orthopedic troubles. β-emitting radionuclides can be used for various joints based on radiation energy. 90Y is a pure β-emitter with a half-life of 64.1 hours that is used for treatment of the knee joint. β-radiation of this radionuclide possesses maximum energy of 2.281 MeV (99.98%), mean pathway of 3.6 mm in the soft tissue and maximum 11 mm. In this project, hydroxyapatite (HAp) is applied as a colloid maker agent that interacts with 90Y3+ ions via ion-dipole bonds and produces 90Y-HAp. The colloidal pharmaceutical is produced by adding an acidic solution of 90YCl3 to an HAp suspension in saline. Effective parameters within which the colloid is applied, such as the volume of diluent, HAp particle size and sonication effect were evaluated and tested. First, these determinative parameters were optimized in the simulated conditions and then examined in the active phase. Finally, the best procedure was determined for the production of the radiopharmaceutical. Radionuclide purity of the radiopharmaceutical according to the primary 90YCl3 solution was over 99.9%. Labeling yield and radiochemical purity were obtained over 99% using TLC method in saline solvent up to three days after production of radiopharmaceutical. Radiochemical purity of 90Y-HAp colloid was also evaluated in human serum albumin solution for three days at room temperature. The amount of released activity was between 0.3 to 2%.

Highlights

  1. G. Modder, Radiosynoviorthesis-involvement of nuclear medicine in rheumatology and orthopaedics, Germany: Warlich Druck und Verlagsges (1995).

     

  2. B.K. DAS, P.K. Pradhan, A.K. Shukla, R. Misra, Role of radiosynovectomy in rheumatoid arthritis, J. Indian Rheumatol Assoc, 12 (2004) 98-103.

 

  1. O.T. Makela, M.J. Lammi, H. Usitalo, P. Penttila, E. Kolehmainen, A. Sukura, S. Sankari, R.M. Tulamo, Experimental radiation synovectomy in rabbit knee with holmium-166 ferric hydroxide macro aggregate, Nucl. Med. Biol, 29 (2002) 593-598.

 

  1. S. Ofluoglu, E. Schwameis, H. Zehetgruber, E. Havlik, A. Wanivenhaus, I. Schweeger, K. Weiss, H. Sinzinger, C. Pirich, Radiation synovectomy with (166) Ho-ferric hydroxide: a first experience, J. Nucl. Med, 43 (2002) 1489-1494.

 

  1. H.J. Siegel, J.V. Jr Lock, M.E. Siegel, C. Quinones, Phosphate-32 colloid radiosynovectomy in hemophilia: outcome of 125 procedures, Clin. Orthop. Relat. Res, 392 (2001) 409-417.

 

  1. K. Kothari, S. Suresh, H.D. Sarma, V. Meera, M.R.A. Pillai, 188Re-labeled hydroxyapatite particles for radiation synovectomy, Appl. Radiat. Isot, 58 (2003) 463-468.

 

  1. C.Y. Shin, M. Son, J.I. Ko, M.Y. Jung, I.K. Lee, S.H. Kim, W.B. Kim, J.M. Jeong, Y.W. Song, DA-7911, 188-Rheniumtin colloid, as a new therapeutic agent of rheumatoid arthritis, Arch. Pharm. Res, 26 (2003) 168-172.

  2. B.C. Shin, K.B. Park, B.S. Jang, S.M. Lim, C.K. Shim, Preparation of 153Sm-chitosan complex for radiation synovectomy, Nucl. Med. Biol, 28 (2001) 719-725.

 

  1. S. Srivastava, E. Dadachova, Recent advances in radionuclide therapy, Semin. Nucl. Med, 31 (2001) 330-341.

 

  1.  C. Lueders, M. Kopec, K. Morstin, T. Schmitz, L.E. Feinendegen, Die radiosynoviorthese anwendung und durchfuhrung unter Besonderer Berucksitigung dosimetrischer aspekte, Akt. Rheumatol, 17 (1992) 74-81.

 

  1.  W.M. Baker, The formation of abnormal synovial cysts in connection with the joints, St Bartholomew’s Hosp. Rep, 21 (1885) 177-190.

 

  1.  U. Pandey, K. Bapat, H. Dev Sarma, P.S. Dhami, P.W. Naik, G. Samuel, M. Venkatesh, Bioevaluation of 90Y-labeled particles in animal model of arthritis, Ann. Nucl. Med, 23 (2009) 333-339.

 

  1.  P. Schneider, J. Farahati, C. Reiners, Radiosynovectomy in rheumatology, orthopedics, and hemophilia, J. Nucl. Med, 46 (2005) 48S-54S.

 

  1.  F.W.S. Webb, J. Lowe, R. Bluestone, Uptake of colloidal radioactive yttrium by synovial membrane, Ann. Rheum. Dis., 28 (1969) 300-302.

 

  1.  A.M. Isomaki, H. Inouhe, M. Oka, Uptake of 90-Yttrium resin colloid by synovial cells and synovial membrane in rheumatoid arthritis, Scand. J. Rheum, 1 (1972) 53-60.

     

  2.  M. Khalid, A. Mushtaq, Preparation and in vitro stability of (n,γ) Yttrium-90 hydrony apatite, Appl. Radiat. Isot, 62 (2005) 587-590.

 

  1.  J.M. Hughes, M. Cameron, K.D. Crowley, Structural variations in natural F, OH, and Cl apatites, American Mineralogist, 74 (1989) 870-876.

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

  2.  Gopal B. Saha, Fundamentals of Nuclear Pharmacy, 5th Ed., Springer-Verlag NY, Translated by: M. Ghannadi Maragheh, R. Gholipour Peyvandi and A. Bahrami Samani (2003).

 

 R. LeGeros, Biologically relevant calcium phosphates: preparation and characterization, calcium phosphates in oral biology and medicine, Basel: Karger, 31 (1991).

Keywords

Yttrium-90
Hydroxyapatite
Colloidal Radiopharmaceutical
90Y-HAp
Radiosynovectomy

  1. G. Modder, Radiosynoviorthesis-involvement of nuclear medicine in rheumatology and orthopaedics, Germany: Warlich Druck und Verlagsges (1995).

     

  2. B.K. DAS, P.K. Pradhan, A.K. Shukla, R. Misra, Role of radiosynovectomy in rheumatoid arthritis, J. Indian Rheumatol Assoc, 12 (2004) 98-103.

 

  1. O.T. Makela, M.J. Lammi, H. Usitalo, P. Penttila, E. Kolehmainen, A. Sukura, S. Sankari, R.M. Tulamo, Experimental radiation synovectomy in rabbit knee with holmium-166 ferric hydroxide macro aggregate, Nucl. Med. Biol, 29 (2002) 593-598.

 

  1. S. Ofluoglu, E. Schwameis, H. Zehetgruber, E. Havlik, A. Wanivenhaus, I. Schweeger, K. Weiss, H. Sinzinger, C. Pirich, Radiation synovectomy with (166) Ho-ferric hydroxide: a first experience, J. Nucl. Med, 43 (2002) 1489-1494.

 

  1. H.J. Siegel, J.V. Jr Lock, M.E. Siegel, C. Quinones, Phosphate-32 colloid radiosynovectomy in hemophilia: outcome of 125 procedures, Clin. Orthop. Relat. Res, 392 (2001) 409-417.

 

  1. K. Kothari, S. Suresh, H.D. Sarma, V. Meera, M.R.A. Pillai, 188Re-labeled hydroxyapatite particles for radiation synovectomy, Appl. Radiat. Isot, 58 (2003) 463-468.

 

  1. C.Y. Shin, M. Son, J.I. Ko, M.Y. Jung, I.K. Lee, S.H. Kim, W.B. Kim, J.M. Jeong, Y.W. Song, DA-7911, 188-Rheniumtin colloid, as a new therapeutic agent of rheumatoid arthritis, Arch. Pharm. Res, 26 (2003) 168-172.

  2. B.C. Shin, K.B. Park, B.S. Jang, S.M. Lim, C.K. Shim, Preparation of 153Sm-chitosan complex for radiation synovectomy, Nucl. Med. Biol, 28 (2001) 719-725.

 

  1. S. Srivastava, E. Dadachova, Recent advances in radionuclide therapy, Semin. Nucl. Med, 31 (2001) 330-341.

 

  1.  C. Lueders, M. Kopec, K. Morstin, T. Schmitz, L.E. Feinendegen, Die radiosynoviorthese anwendung und durchfuhrung unter Besonderer Berucksitigung dosimetrischer aspekte, Akt. Rheumatol, 17 (1992) 74-81.

 

  1.  W.M. Baker, The formation of abnormal synovial cysts in connection with the joints, St Bartholomew’s Hosp. Rep, 21 (1885) 177-190.

 

  1.  U. Pandey, K. Bapat, H. Dev Sarma, P.S. Dhami, P.W. Naik, G. Samuel, M. Venkatesh, Bioevaluation of 90Y-labeled particles in animal model of arthritis, Ann. Nucl. Med, 23 (2009) 333-339.

 

  1.  P. Schneider, J. Farahati, C. Reiners, Radiosynovectomy in rheumatology, orthopedics, and hemophilia, J. Nucl. Med, 46 (2005) 48S-54S.

 

  1.  F.W.S. Webb, J. Lowe, R. Bluestone, Uptake of colloidal radioactive yttrium by synovial membrane, Ann. Rheum. Dis., 28 (1969) 300-302.

 

  1.  A.M. Isomaki, H. Inouhe, M. Oka, Uptake of 90-Yttrium resin colloid by synovial cells and synovial membrane in rheumatoid arthritis, Scand. J. Rheum, 1 (1972) 53-60.

     

  2.  M. Khalid, A. Mushtaq, Preparation and in vitro stability of (n,γ) Yttrium-90 hydrony apatite, Appl. Radiat. Isot, 62 (2005) 587-590.

 

  1.  J.M. Hughes, M. Cameron, K.D. Crowley, Structural variations in natural F, OH, and Cl apatites, American Mineralogist, 74 (1989) 870-876.

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

  2.  Gopal B. Saha, Fundamentals of Nuclear Pharmacy, 5th Ed., Springer-Verlag NY, Translated by: M. Ghannadi Maragheh, R. Gholipour Peyvandi and A. Bahrami Samani (2003).

 

 R. LeGeros, Biologically relevant calcium phosphates: preparation and characterization, calcium phosphates in oral biology and medicine, Basel: Karger, 31 (1991).

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