Document Type : Research Paper

Authors

• M Salouti
• M.H Babaei
• H Rajabi
• H Forootan
• M.J Rasaee
• A Bitarafan Rajabi
• F Johari Daha
• M Mazidi
• J Mohammad Nejad
• M Shafiee

Abstract

Immunoglobulin fragments offer a number of advantages for intratumor penetration over the intact antibodies. The goal of this study was to prepare F(ab')₂ fragmants from antibody PR81 and ^99mTc labeling in order to produce a biological radiopharmaceutical for imaging of breast cancer in nuclear medicine. The antibody PR81 was digested with 5% (w/w) pepsin at different times to find the best time for a complete digestion. The prepared F(ab')₂ fragments were purified by protein A column chromatography. The immunoreactivity of the complex assessed by radioimmunoassay was determined to be 65.2% ± 5.1. ^99mTc radiolabeling of F(ab')₂ fragment by using HYNIC as a chelator and tricine as a
co-ligand resulted in a radiochemical purity of 70.1%±5.2 after 1 hr with a high stability. The complex was purified by the gel chromatography method to increase the radiochemical purity. Biodistribution and imaging studies in BALB/c mice with breast tumor xenograft demonstrated as a specific localization of the compound at the site of tumors 4 h post injection with high sensitivity and minimum accumulation in non target organs. The results showed that F(ab')₂ fragment of PR81 is a promising candidate for radioimmunoscintigraphy of the human breast carcinoma.

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Keywords

• Breast Carcinoma
• Radioimmunoscintigraphy
• Antibody PR81
• F(ab′)2
• Fragment
• 99mTc Labeling