Journal of Nuclear Science, Engineering and Technology (JONSAT)
In cooperation with the Iranian Nuclear Society

Optimization of [67Ga]-Oxinate Complex Formation Conditions for white Blood Cell Labeling

Document Type : Research Paper

Authors

A.R Jalilian
A Vakilli
S Nazari
F Bolourinovin
S Rajabifar
Abstract
In this work, the effective factors on the preparation of 67Ga-oxinate complex for white blood cell labeling were determined. Gallium-67 was produced at AMIRS 30MeV cyclotron via 68Zn(p,2n)67Ga reaction in the from of 67GaCl3, and was used for radiolabeling of oxinate complex at optimized conditions. A mixture of 67GaCl3 (3uL, 200uCi) and ethanolic oxine solution (1mg/ml, 100μl) was evaporated and reacted at 25ºC for 1 h in the presence of NaOAc solution (pH. 5.5). ITLC was performed using a mixture of ammonium actetae and methanol solution (1:1) followed by recording the activity using radio thin layer chromatography scanner. The radiochemical purity of %95.18 at these conditions was obtained (specific activity of 1432 GBq/mmol). Freshly prepared white blood cells were separated from human volunteers and used for labeling by the above mentioned complex at 37ºC. 67Ga-oxinate complex due to it’s lipophilicity and suitable gamma rays is a suitable cell labeling agent and available for blood stem cell and microorganism studies. 

Highlights

 

  1. 1.    C. Bekerman, P.B. Hoffer, J.D. Bitran, “The rules of gallium-67 in the clinical evaluation of cancer,” Semin. Nucl. Med. 14, 296-323 (1984).

 

  1. 2.    J. Ruhlmann, P. Oehr, H.J. Biersach, “Cost effectiveness of PET in oncology,” 195-97 in: PET in oncology Ist edition Springer-Verlage Press, New York (1999).

 

  1. 3.   C.M. Lederer, V.S. Shirly, “Table of Isotopes,” 7th Edition. 211, John Willy and Sons Press. New York (1978).

 

  1. 4.    A.R. Jalilian, P. Rowshanfarzad, M. Sabet, A. Rahiminejad-Kisomi A.A. Rajamand, “Development of [66Ga]oxine complex; a possible PET tracer,” Nukleonika 51, 155-159 (2006).

 

  1. 5.    L. Kostakoglow, J. Leonardo, I. Kujh, “Comparision of fluorine -18 fluorodeoxy glucose positron emission tomography and Ga-67 scintigraphy in evalution of lumphoma,” Cancer 94, 879-888 (2002).

 

  1. 6.   http://www.ambion.com/techilb/append/supp/wbc/html.

Keywords

Gallium-67
Oxinate Complex
Optimization
Cell Labeling

  1.  

    1. 1.    C. Bekerman, P.B. Hoffer, J.D. Bitran, “The rules of gallium-67 in the clinical evaluation of cancer,” Semin. Nucl. Med. 14, 296-323 (1984).

     

    1. 2.    J. Ruhlmann, P. Oehr, H.J. Biersach, “Cost effectiveness of PET in oncology,” 195-97 in: PET in oncology Ist edition Springer-Verlage Press, New York (1999).

     

    1. 3.   C.M. Lederer, V.S. Shirly, “Table of Isotopes,” 7th Edition. 211, John Willy and Sons Press. New York (1978).

     

    1. 4.    A.R. Jalilian, P. Rowshanfarzad, M. Sabet, A. Rahiminejad-Kisomi A.A. Rajamand, “Development of [66Ga]oxine complex; a possible PET tracer,” Nukleonika 51, 155-159 (2006).

     

    1. 5.    L. Kostakoglow, J. Leonardo, I. Kujh, “Comparision of fluorine -18 fluorodeoxy glucose positron emission tomography and Ga-67 scintigraphy in evalution of lumphoma,” Cancer 94, 879-888 (2002).

     

    1. 6.   http://www.ambion.com/techilb/append/supp/wbc/html.

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