Journal of Nuclear Science, Engineering and Technology (JONSAT)
In cooperation with the Iranian Nuclear Society

Biosorption of Strontium from Aqueous Solution by the New Strain of Bacillus sp. Strain GT-83

Document Type : Research Paper

Abstract
An attempt was made to isolate bacterial strains capable of removing strontium biologically. In this study ten different water samples collected from Neydasht spring in the north of Iran and then the bacterial species were isolated from the water samples. The initial screening of a total of 50 bacterial isolates resulted in selection of one strain. The isolated strain showed a maximum adsorption capacity with 55mg strontium/g dry wt. It was tentatively identified as Bacillus sp. according to the morphological and biochemical properties, and called strain GT-83. Our studies indicated that Bacillus sp. GT-83 is able to grow aerobically in the presence of 50mM SrCl2, but its growth was inhibited at high levels of strontium concentrations. The biosorption capacity of Bacillus sp. GT-83 depends strongly on the pH solution. Hence the maximum strontium sorption capacity of Bacillus sp. GT-83 was obtained at pH 10, independent of absence or presence of MgCl2 of different concentrations. Strontium-salt biosorption studies were also performed at this pH values. The equilibrium biosorption of strontium was elevated by increasing the strontium concentration, up to 250mg/l for Bacillus sp. GT-83. The maximum biosorption of strontium was obtained at temperatures in the range of 30-35˚C. The Bacillus sp. GT-83 biosorbed 97mg strontium/g dry wt at 100mg/l initial strontium concentration without MgCl2. When MgCl2 concentration increased to
15%(w/v), these values dropped to 23.6mg strontium/g dry wt at the same conditions. Uptake of strontium within 5 min of incubation was relatively rapid and the absorption continued slowly thereafter.

Highlights

  1. 1.      G.J. Kirk and S. Staunton, “On the predicting the fate of radioactive cesium in soil beneath grassland,” Journal of Soil Science, 40, 71-84 (1989).

 

  1. 2.      B.L. Carson, H.V. Ellis, J.L. McCann, “Toxicology and biological monitoring of metals in humans,” Chelsea, MI: Lewis Publishers (1986).

 

  1. 3.      S. Ghorbanzadeh Mashkani, P. Tajer Mohammad Ghazvini, H. Ghafourian, “Biofiltration of Cs and Sr ions from aqueous solutions by native and chemically treated Azolla filiculoides,” International Journal of Ecological Economics and Statistic. 13, 17-24 (2009).

 

  1. 4.      M. Nourbakhsh, Y. Sag, D. Ozer, Z. Aksu, T. Kustal, A. Caglar, “A comparative study of various biosorbents for removal of Cr(VI), ions from industrial waste waters,” Process Biochemistry. 29, 1-5 (1994).

 

  1. 5.      E. Luef, T. Prey, C.P. Kubicek, “Biosorption of zinc by fungal mycelial wastes,” Applied Microbiology and Biotechnology. 34, 688-692 (1991).

 

  1. 6.      V.V. Panchanodikar, R.P. Das, “Biorecovery of zinc from industrial effluent using native microflora,” International Journal of Environmental Study, 44, 251-257 (1993).

 

  1. 7.      Z. Aksu, Y. Sag, T. Kutsal, “A comparative study of adsorption of chromium (VI) ions to C. vulgaris and Z. ramigera,” Environmental Technology. 11, 33-40 (1990).

 

  1. 8.      N. Kuyucak, B. Volesky, “Biosorbent for recovery of metals from industrial solutions,” Biotechnology Letter, 10 (2), 137-142 (1988).

 

  1. 9.      A. Lopez, N. Lazano, J.M. Priego, A.M. Marqus, “Effect of pH on the biosorption of Nickel and other heavy metals by Pseudomonas fluoresces 4F39,” Journal of Industerial Microbiology and Biotechnology, 24, 146-151 (2000).

 

  1. 10.  N. Goyal, S.C. Jain, U.C. Banerjee, “Comparative studies on the microbial desorption of heavy metals,” Advances in Environmental Research, 7, 311-319 (2003).

 

 

  1. 11.  J.G. Holt, N.R. Krieg, P.H.A. Sneath, J.T Staley, S.T.Williams, “Bergey’s Manual of Systematic Bacteriology,” Vol. 1–4. Williams and Wilkins, Baltimore (1989).

 

  1. 12.  S.T. Cowan, K.J. Steel, G.I. Barrow, R.K.A. Feltham, “Cowan and Steel's Manual for the Identification of Medical Bacteria,” Cambdrige University Press (1993).

 

  1. 13.  S. Ghorbanzadeh Mashkani, F. Malekzadeh, H. Ghafurian, M.R. Soudi, P. Tajer Mohammad Ghazvini, “Biosorption of tungsten from aqueous solutions by new strain of Bacillus sp. MGG-83 isolated from Anzali lagoon,” Journal of Nuclear Science and Technology, 37, 22-28 (2006).
  2. 14.  S. Ghorbanzadeh Mashkani, P. Tajer Mohammad Ghazvini, H. Ghafourian, M.A. Ahmadi, “Biosorption of rhenium by new strain of Bacillus sp. MGG-83,” Journal of Nuclear Science and Technology, 38, 31-35 (2007).

 

P. Tajer Mohammad Ghazvini, S. Ghorbanzadeh Mashkani, “Phytoremediation of aqueous solutions polluted by Cr(VI) and Pb(II) by Azolla: A new bioseparation process for wastewater treatment,” 10th International Conference on Environmental Science and Technology, Cosisland, Greece, September 5th - 7th, A-410- A-416 (2007).

Keywords

Bacillus sp. GT-83
Biosorption
Neydasht Spring
Radionuclide
Strontium
Wastewater
  1. 1.      G.J. Kirk and S. Staunton, “On the predicting the fate of radioactive cesium in soil beneath grassland,” Journal of Soil Science, 40, 71-84 (1989).

 

  1. 2.      B.L. Carson, H.V. Ellis, J.L. McCann, “Toxicology and biological monitoring of metals in humans,” Chelsea, MI: Lewis Publishers (1986).

 

  1. 3.      S. Ghorbanzadeh Mashkani, P. Tajer Mohammad Ghazvini, H. Ghafourian, “Biofiltration of Cs and Sr ions from aqueous solutions by native and chemically treated Azolla filiculoides,” International Journal of Ecological Economics and Statistic. 13, 17-24 (2009).

 

  1. 4.      M. Nourbakhsh, Y. Sag, D. Ozer, Z. Aksu, T. Kustal, A. Caglar, “A comparative study of various biosorbents for removal of Cr(VI), ions from industrial waste waters,” Process Biochemistry. 29, 1-5 (1994).

 

  1. 5.      E. Luef, T. Prey, C.P. Kubicek, “Biosorption of zinc by fungal mycelial wastes,” Applied Microbiology and Biotechnology. 34, 688-692 (1991).

 

  1. 6.      V.V. Panchanodikar, R.P. Das, “Biorecovery of zinc from industrial effluent using native microflora,” International Journal of Environmental Study, 44, 251-257 (1993).

 

  1. 7.      Z. Aksu, Y. Sag, T. Kutsal, “A comparative study of adsorption of chromium (VI) ions to C. vulgaris and Z. ramigera,” Environmental Technology. 11, 33-40 (1990).

 

  1. 8.      N. Kuyucak, B. Volesky, “Biosorbent for recovery of metals from industrial solutions,” Biotechnology Letter, 10 (2), 137-142 (1988).

 

  1. 9.      A. Lopez, N. Lazano, J.M. Priego, A.M. Marqus, “Effect of pH on the biosorption of Nickel and other heavy metals by Pseudomonas fluoresces 4F39,” Journal of Industerial Microbiology and Biotechnology, 24, 146-151 (2000).

 

  1. 10.  N. Goyal, S.C. Jain, U.C. Banerjee, “Comparative studies on the microbial desorption of heavy metals,” Advances in Environmental Research, 7, 311-319 (2003).

 

 

  1. 11.  J.G. Holt, N.R. Krieg, P.H.A. Sneath, J.T Staley, S.T.Williams, “Bergey’s Manual of Systematic Bacteriology,” Vol. 1–4. Williams and Wilkins, Baltimore (1989).

 

  1. 12.  S.T. Cowan, K.J. Steel, G.I. Barrow, R.K.A. Feltham, “Cowan and Steel's Manual for the Identification of Medical Bacteria,” Cambdrige University Press (1993).

 

  1. 13.  S. Ghorbanzadeh Mashkani, F. Malekzadeh, H. Ghafurian, M.R. Soudi, P. Tajer Mohammad Ghazvini, “Biosorption of tungsten from aqueous solutions by new strain of Bacillus sp. MGG-83 isolated from Anzali lagoon,” Journal of Nuclear Science and Technology, 37, 22-28 (2006).
  2. 14.  S. Ghorbanzadeh Mashkani, P. Tajer Mohammad Ghazvini, H. Ghafourian, M.A. Ahmadi, “Biosorption of rhenium by new strain of Bacillus sp. MGG-83,” Journal of Nuclear Science and Technology, 38, 31-35 (2007).

 

P. Tajer Mohammad Ghazvini, S. Ghorbanzadeh Mashkani, “Phytoremediation of aqueous solutions polluted by Cr(VI) and Pb(II) by Azolla: A new bioseparation process for wastewater treatment,” 10th International Conference on Environmental Science and Technology, Cosisland, Greece, September 5th - 7th, A-410- A-416 (2007).

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