Biosorption of Tungsten from Aqueous Solutions by New Strain of Bacillus sp. MGG-83 Isolated from Anzali Lagoon

Ghorbanzadeh Mashkani, S; Malekzadeh, F; Ghafourian, H; Soudi, M.R; Tajer Mohammad Ghazvini, P

Biosorption of Tungsten from Aqueous Solutions by New Strain of Bacillus sp. MGG-83 Isolated from Anzali Lagoon

Document Type : Research Paper

Authors

S Ghorbanzadeh Mashkani

F Malekzadeh

H Ghafourian

M.R Soudi

P Tajer Mohammad Ghazvini

Abstract

In this study 38 water and sludge samples were collected from various areas of Anzali lagoon and 100 strains were isolated when the samples were cultured in the media. Among them, only one strain showed the maximum absorption capacity (65.4 mg/g dry wt). It was tentatively called MGG-83 strain. Further investigations proved that the strain MGG-83 belonged to the genus of Bacillus. The maximum uptake of tungsten was obtained at pH 2. The tungsten taken up by the Bacillus sp. MGG-83 increased by increasing the concentration of the tungsten. At 600mg/l, the uptake was 545.6 mg/g dry wt. The uptake of tungsten within 5 min of incubation was relatively rapid and the absorption continued slowly thereafter. When sodium azide and 2,4 dinitrophenol were used, it was evident the 80% of the absorbed tungsten is independent of the metabolism and 20% depends on the metabolism. When the cells were treated by the heat, the tungsten uptake decreased to 11%. The tungsten uptake increased with increasing in biomass of dry weight of the cells (87% at 20 mg biomass; 100% at 25mg biomass). The applied temperature in the range of 20-45⁰C did not show any significant change in the uptake.

Highlights

1. N.P. L’vov, A.N. Nosikov, A.N. Antipov, “Tungsten containing enzymes,” Biochemistry (Moscow). 67, 196-200 (2002).

2. H.T. Evans, F.T. Manheim, S. Landegren, Molybdenum. In: “Handbook of Geochemistry,” (Wedenpohl, K.H., Ed.), Vol.11/5, Chapter 42. Springer Verlag, Berlin, New York (1974).

3. M.J. O'Neil, A. Smith, P.E. Heckelman, In: “The Merck index. An encyclopedia of chemicals, drugs, and biologicals,” Whitehouse Station, NJ: Merck Research Laboratories, 1748 (2001).

4. J. Vucina and R. Han , “Production and therapeutic use of rhenium-186, 188 the future of radionuclides,” Med Pregl. 56, 362-365 (2003).

5. W. Sahle, S. Krantz, B. Christensson, “Preliminary data on hard metal workers exposed to tungsten oxide fibers,” Sci Total Environ. 191, 153-167 (1996).

6. L.P. Wackett, A.G. Dodge, L.B.M Ellis, “Microbial Genomics and the Periodic Table,” Appl and Environ Microbiol. 70, 647-655 (2004).

7. D.H. Nies, “Microbial heavy-metal resistance,” Appl Microbiol Biotechnol. 5, 730-750 (1999).

8. A.I. Zouboulis, M.X. Loukidou, K.A. Matis, “Biosorption of toxic metals from aqueous solutions by bacteria strains isolated from metal-polluted soils,” Process Biochemistry. 39, 909-916 (2004).

9. B. Volesky, “Biosorbent Materials,” Biotechnol. Bioeng. Symp. 16,121-126 (1986).

10. N. Liu, J. Liao, S. Luo, Y. Yang, J. Jin, T. Zhang, P. Zhao, “Biosorption of ²⁴¹Am by immobilized Saccharomyces cerevisiae,” J. Radioanalytical and Nuclear Chemistry. 258, 59-63 (2003).

11. J.G. Holt, N.R. Krieg, P.H.A. Sneath, J.T Staley, S.T. Williams, “Bergey’s Manual of Systematic Bacteriology”, Vol. 1–4. Williams and Wilkins, Baltimore (1989).

12. E.P. Welsch, “A rapid geochemical spectrophotometric determination of tungsten with dithiol,” Talanta. 30, 876-878 (1983).

13. A. Lopez, N. Lazano, J.M. Priego, A.M. Marqus, “Effect of pH on the biosorption of Nickel and other heavy metals by Pseudomonas fluoresces 4F39,” J. Industerial. Microbiol & Biotechnol. 24, 146-151 (2000).

14. A.M. Marqus, X. Roca, M.D. Simon-Pujuol, M.C. Fuste, F. Congregado, “Uranium accumulation by Pseudomonas sp. EPS 5028,” Appli. Microbiol. Biotechnol. 35, 406-410 (1991).

15. M.P. Pons and M.C. Fuste, “Uranium uptake by immobilized cell of Pseudomonas strain EPS 5028,” Appli. Microbiol. Biotechnol. 39, 661-665 (1993).

N. Goyal, S.C. Jain, U.C. Banerjee, “Comparative studies on the microbial adsorption of heavy metals,” Advances in Environmental Research 17-7, 311-319 (2003).

Keywords

Biosorption

Tungsten

Bacillus sp

Heavy metals

Anzali Lagoon

1. N.P. L’vov, A.N. Nosikov, A.N. Antipov, “Tungsten containing enzymes,” Biochemistry (Moscow). 67, 196-200 (2002).

2. H.T. Evans, F.T. Manheim, S. Landegren, Molybdenum. In: “Handbook of Geochemistry,” (Wedenpohl, K.H., Ed.), Vol.11/5, Chapter 42. Springer Verlag, Berlin, New York (1974).

3. M.J. O'Neil, A. Smith, P.E. Heckelman, In: “The Merck index. An encyclopedia of chemicals, drugs, and biologicals,” Whitehouse Station, NJ: Merck Research Laboratories, 1748 (2001).

4. J. Vucina and R. Han , “Production and therapeutic use of rhenium-186, 188 the future of radionuclides,” Med Pregl. 56, 362-365 (2003).

5. W. Sahle, S. Krantz, B. Christensson, “Preliminary data on hard metal workers exposed to tungsten oxide fibers,” Sci Total Environ. 191, 153-167 (1996).

6. L.P. Wackett, A.G. Dodge, L.B.M Ellis, “Microbial Genomics and the Periodic Table,” Appl and Environ Microbiol. 70, 647-655 (2004).

7. D.H. Nies, “Microbial heavy-metal resistance,” Appl Microbiol Biotechnol. 5, 730-750 (1999).

8. A.I. Zouboulis, M.X. Loukidou, K.A. Matis, “Biosorption of toxic metals from aqueous solutions by bacteria strains isolated from metal-polluted soils,” Process Biochemistry. 39, 909-916 (2004).

9. B. Volesky, “Biosorbent Materials,” Biotechnol. Bioeng. Symp. 16,121-126 (1986).

11. J.G. Holt, N.R. Krieg, P.H.A. Sneath, J.T Staley, S.T. Williams, “Bergey’s Manual of Systematic Bacteriology”, Vol. 1–4. Williams and Wilkins, Baltimore (1989).

12. E.P. Welsch, “A rapid geochemical spectrophotometric determination of tungsten with dithiol,” Talanta. 30, 876-878 (1983).

13. A. Lopez, N. Lazano, J.M. Priego, A.M. Marqus, “Effect of pH on the biosorption of Nickel and other heavy metals by Pseudomonas fluoresces 4F39,” J. Industerial. Microbiol & Biotechnol. 24, 146-151 (2000).

14. A.M. Marqus, X. Roca, M.D. Simon-Pujuol, M.C. Fuste, F. Congregado, “Uranium accumulation by Pseudomonas sp. EPS 5028,” Appli. Microbiol. Biotechnol. 35, 406-410 (1991).

15. M.P. Pons and M.C. Fuste, “Uranium uptake by immobilized cell of Pseudomonas strain EPS 5028,” Appli. Microbiol. Biotechnol. 39, 661-665 (1993).

N. Goyal, S.C. Jain, U.C. Banerjee, “Comparative studies on the microbial adsorption of heavy metals,” Advances in Environmental Research 17-7, 311-319 (2003).