DETECTION OF DNA COMET BY USING OF SCGE FOR IRRADIATED POULTRY AND SHRIMP

Rajaie, R; Hosseini, S.L

DETECTION OF DNA COMET BY USING OF SCGE FOR IRRADIATED POULTRY AND SHRIMP

Document Type : Research Paper

Authors

R Rajaie

S.L Hosseini

Abstract

DNA in food will sustain damage by gamma radiation that this damage was detected by a sensetive technique called single cell gel electrophoresis. For this purpose poultry and shrimp samples were irradiated by ⁶⁰Co gamma radiation. The radiation doses for poultry were 2, 5 and 7 kGy and for shrimp were 3 and 7 kGy. Then, irradiated samples were compared with unirradiated ones (control). In addition, the effects of shelf-life and temperature were considered on the same samples. We found that with different doses (even at low dose( identification of irradiated from unirradiated samples were easily possible.

Highlights

H. Delincee, “Detection of food treated with ionizing radiation,” Trends in Food Science and Technology, 9, 73-82 (1998).
M. Makoto et.al., “Capability for identification of gamma irradiated bovine liver by new high sensivity comet assay,” Biol.Pharm.Bull. 23 (12), 1399-1405 (2000).
G. Koppen and H. Cerda, “identification of low-dose irradiated seeds using the neutral comet assay,” Lebensm.-Wiss. u.-Thenol. 30, 452-457 (1997).
P.L. Olive, “DNA damage and repair in individual cells: applications of the comet assay in radiobiology,” Int. J. Radiat. Biol. 75 (4), 395-405 (1999).
P.L. Olive et.al., “DNA double-strand breaks measured inindividual cells subjected to gel electrophoresis,” Cancer Research 51, 4671-4676 (1991).
P.L. Olive, “The role of DNA single- and double-strand breaks in cell killing by ionizing radiation,” Radiation Research 150, S42-S51 (1998).
P.L. Olive et.al., “Factors influencing DNA migration from individual cells subjected to gel electrophoresis,” Experimental Cell Research 198, 256-267 (1992).
S. Cotelle and J.F. Ferard, “Comet assay in genetic ecotoxicology: A rewiew,” Environ. Molecul. Mutagen. 34, 246-255 (1999).
A.R. Collins et.al., “ The comet assay: What can it really tell us?,” Mutation Research, 375, 183-193 (1997).
H. Delincee,“Rapid and simple screening tests to detect the radiation treatment of foods,” Radiat. Phys. Chem. 46 (4-6), 677-680 (1995).
H. Cerda, H. Delincee, H. Haine and H. Rupp “The DNA comet assay as a rapid screening technique to control irradiated food,” Mutation Research 375, 167-181 (1997).
H. Delincee, “Silver staining of DNA in the comet assay,” Comet Newsletter (1995).
S.M. Piperakis et.al., “Comet assay for nuclear DNA damage,” Methods in Enzymology, 300, 184-194 (1999).
N.P. Singh et.al., “A simple technique for quantitation of low levels of DNA damage in individual cells,” Experimental Cell Research, 175, 184-191 (1988).
Z. Szot et.al., “Effect of storage conditions and gamma irradiation of meat on DNA analyzed by comet assay,” Nuclear Technologies and Methods, 105-107 (1999).

16. Comet Newsletter, Issue#6, April (1997).

17. Codex Alimentarius, Volume XV, Rome (1984).

18. Food Irradiation newsletter, Supplement (1995).

Keywords

poultry

shrimp

irradiation

single cell gel electrophoresis

DNA comet assay

H. Delincee, “Detection of food treated with ionizing radiation,” Trends in Food Science and Technology, 9, 73-82 (1998).
M. Makoto et.al., “Capability for identification of gamma irradiated bovine liver by new high sensivity comet assay,” Biol.Pharm.Bull. 23 (12), 1399-1405 (2000).
G. Koppen and H. Cerda, “identification of low-dose irradiated seeds using the neutral comet assay,” Lebensm.-Wiss. u.-Thenol. 30, 452-457 (1997).
P.L. Olive, “DNA damage and repair in individual cells: applications of the comet assay in radiobiology,” Int. J. Radiat. Biol. 75 (4), 395-405 (1999).
P.L. Olive et.al., “DNA double-strand breaks measured inindividual cells subjected to gel electrophoresis,” Cancer Research 51, 4671-4676 (1991).
P.L. Olive, “The role of DNA single- and double-strand breaks in cell killing by ionizing radiation,” Radiation Research 150, S42-S51 (1998).
P.L. Olive et.al., “Factors influencing DNA migration from individual cells subjected to gel electrophoresis,” Experimental Cell Research 198, 256-267 (1992).
S. Cotelle and J.F. Ferard, “Comet assay in genetic ecotoxicology: A rewiew,” Environ. Molecul. Mutagen. 34, 246-255 (1999).
A.R. Collins et.al., “ The comet assay: What can it really tell us?,” Mutation Research, 375, 183-193 (1997).
H. Delincee,“Rapid and simple screening tests to detect the radiation treatment of foods,” Radiat. Phys. Chem. 46 (4-6), 677-680 (1995).
H. Cerda, H. Delincee, H. Haine and H. Rupp “The DNA comet assay as a rapid screening technique to control irradiated food,” Mutation Research 375, 167-181 (1997).
H. Delincee, “Silver staining of DNA in the comet assay,” Comet Newsletter (1995).
S.M. Piperakis et.al., “Comet assay for nuclear DNA damage,” Methods in Enzymology, 300, 184-194 (1999).
N.P. Singh et.al., “A simple technique for quantitation of low levels of DNA damage in individual cells,” Experimental Cell Research, 175, 184-191 (1988).
Z. Szot et.al., “Effect of storage conditions and gamma irradiation of meat on DNA analyzed by comet assay,” Nuclear Technologies and Methods, 105-107 (1999).