Journal of Nuclear Science, Engineering and Technology (JONSAT)
In cooperation with the Iranian Nuclear Society

Feasibility Study for Separation of Stable and Radioisotope of Molybdenum Isotopes with Biofilter Using Microorganisms

Document Type : Research Paper

Authors

H Ghafourian
M Shams Rafiee
M Mazaheri Tehrani
M.A Ahmadi
A Nafar
Abstract
In this study 23 bacterial strains were isolated, purified and investigated from samples of radioactive polluted water collected from different areas in Ramsar, soils of Hormoz Island, copper and molybdenum concentrated and various places of Copper Sarcheshmeh Mines. Among the isolated strains from samples that were investigated, isolated strains from soils of Copper Sarcheshmeh Mines showed their growth ability at the environment up to 1000 ppm molybdenum and presented a more adsorption ability of molybdenum in relation to other strains. In the pH adsorption study, the optimum value of pH =4 was selected. The effect of Mo-concentration in the adsorption process with 50 to 1000 ppm of Mo was investigated and the results shown that the highest adsorption is up to 200 ppm with 40% of molybdenum. In dynamics experiments of purified strains from soil samples of Copper Sarcheshmeh Mines showed that the biomasses were saturated almost 95% within 30 minutes. The average dry weight of bacterias were 0.1-0.7 gr per liter of environment and the adsorption capacity of biomasses varied from 50 to 304 mg Mo gr-1 dry biomasses. In comparison with the adsorption of resisting strains in high concentration of molybdenum with primary isolated strains were definite that the primary strains of 26-   









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تاریخ دریافت مقاله: 29/7/1381      تاریخ پذیرش مقاله: 21/3/1382     *email: GHAFORIAN@seai.neda.net.ir                                                 90% and resisting strains of 3-77% removed the molybdenum from 200 ppm. Therefore, the primary strains presented the higher adsorption capacity from the existing molybdenum which proved the occupation of some molybdenum adsorption sites on the surface of resisting bacterias cell on the adaptation time. Although there is still no proper definition about the mechanism of molybdenum adsorption, but by consideration of the optimum pH adsorption, we should state that the relative strains would mainly adsorb the kinds of polymeric anion and molybdate ions. The goal in the first stage of this research was to study the scope of molybdenum adsorption by isolated strains from soils of Copper Sarcheshmeh Mines and optimization of adsorption situation in the direction of providing radioisotope of molybdenum, which will be used in the nuclear medicine for diagnostic.

Highlights

  1. G. M. Gadd, “Uptake of heavy metals,” Biotechnology, 6, 401-403 (1986).

     

  2. C. White and G. M. Gadd, “Heavy metal and radionuclide uptake by fungi and yeast,” J. Chem. Technol. Biotechnol. 49, 331-43 (1990).

     

  3. M. Tsezos and B. Volesky, “Biosorption of Uranium and Thorium,” Biotech. Bioeng. 24, 385-401 (1982).

     

  4. J. M. Tobin and D. Cooper, “Uptake of Metalions by Rhizopus arrhizus biomass,” Appl. Environ. Microbiol. 47, 821-824 (1984).

     

  5. D. S. Wales and B. F. Sagar, “Removal and recovery of heavy metals by biosorption,” J. Chem. Technol. Biotech. 49, 345-55 (1980).

  6. J. A. Brierley and G. M. Goyak, “Metal recovery,” U. S. Patent 4, 789-481 (1988).

     

  7. Nabil Hafez, Alaa S. Abdel - Razek, “Accumulation of some heavy metals on Aspergillus flavus,” J. Chem. Tech. Biotechnol 68, 19-22 (1997).

     

  8. B. Volesky and Z. R. Holan, “Biosorption of Heavy Metals,” Biotechnol. Prog. 11, 235-250 (1995).

                                            

  9. T. Sakaguchi and A. Nacajima, “Studies on the accumulation of heavy metals in biological systems,” Eur. J. Appl. Microbiol. Biotechnol. 12, 84-89 (1981).

Keywords

microorganism
biofilter
molybdenum isotope
radioactive polluted water

  1. G. M. Gadd, “Uptake of heavy metals,” Biotechnology, 6, 401-403 (1986).

     

  2. C. White and G. M. Gadd, “Heavy metal and radionuclide uptake by fungi and yeast,” J. Chem. Technol. Biotechnol. 49, 331-43 (1990).

     

  3. M. Tsezos and B. Volesky, “Biosorption of Uranium and Thorium,” Biotech. Bioeng. 24, 385-401 (1982).

     

  4. J. M. Tobin and D. Cooper, “Uptake of Metalions by Rhizopus arrhizus biomass,” Appl. Environ. Microbiol. 47, 821-824 (1984).

     

  5. D. S. Wales and B. F. Sagar, “Removal and recovery of heavy metals by biosorption,” J. Chem. Technol. Biotech. 49, 345-55 (1980).

  6. J. A. Brierley and G. M. Goyak, “Metal recovery,” U. S. Patent 4, 789-481 (1988).

     

  7. Nabil Hafez, Alaa S. Abdel - Razek, “Accumulation of some heavy metals on Aspergillus flavus,” J. Chem. Tech. Biotechnol 68, 19-22 (1997).

     

  8. B. Volesky and Z. R. Holan, “Biosorption of Heavy Metals,” Biotechnol. Prog. 11, 235-250 (1995).

                                            

  9. T. Sakaguchi and A. Nacajima, “Studies on the accumulation of heavy metals in biological systems,” Eur. J. Appl. Microbiol. Biotechnol. 12, 84-89 (1981).

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