Document Type : Research Paper

Authors

• A.R Jalilian
• A Vakilli
• S Nazari
• F Bolourinovin
• S Rajabifar

Abstract

In this work, the effective factors on the preparation of ⁶⁷Ga-oxinate complex for white blood cell labeling were determined. Gallium-67 was produced at AMIRS 30MeV cyclotron via ⁶⁸Zn(p,2n)⁶⁷Ga reaction in the from of ⁶⁷GaCl₃, and was used for radiolabeling of oxinate complex at optimized conditions. A mixture of ⁶⁷GaCl₃ (3uL, 200uCi) and ethanolic oxine solution (1mg/ml, 100μl) was evaporated and reacted at 25^ºC for 1 h in the presence of NaOAc solution (pH. 5.5). ITLC was performed using a mixture of ammonium actetae and methanol solution (1:1) followed by recording the activity using radio thin layer chromatography scanner. The radiochemical purity of %95.18 at these conditions was obtained (specific activity of 1432 GBq/mmol). Freshly prepared white blood cells were separated from human volunteers and used for labeling by the above mentioned complex at 37^ºC. ⁶⁷Ga-oxinate complex due to it’s lipophilicity and suitable gamma rays is a suitable cell labeling agent and available for blood stem cell and microorganism studies. 

Highlights

 

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Keywords

• Gallium-67
• Oxinate Complex
• Optimization
• Cell Labeling