نوع مقاله : مقاله پژوهشی
نویسندگان
1 پژوهشکده تحقیقات کشاورزی، پزشکی و صنعتی، پژوهشگاه علوم و فنون هسته‌ای، سازمان انرژی اتمی ایران، صندوق پستی: 498-31485، کرج- ایران
2 دانشکده فنی و مهندسی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، صندوق پستی: 775-14515، تهران - ایران
کلیدواژه‌ها
عنوان مقاله English
نویسندگان English
In this work, the effective factors on the preparation of 67Ga-oxinate complex for white blood cell labeling were determined. Gallium-67 was produced at AMIRS 30MeV cyclotron via 68Zn(p,2n)67Ga reaction in the from of 67GaCl3, and was used for radiolabeling of oxinate complex at optimized conditions. A mixture of 67GaCl3 (3uL, 200uCi) and ethanolic oxine solution (1mg/ml, 100μl) was evaporated and reacted at 25ºC for 1 h in the presence of NaOAc solution (pH. 5.5). ITLC was performed using a mixture of ammonium actetae and methanol solution (1:1) followed by recording the activity using radio thin layer chromatography scanner. The radiochemical purity of %95.18 at these conditions was obtained (specific activity of 1432 GBq/mmol). Freshly prepared white blood cells were separated from human volunteers and used for labeling by the above mentioned complex at 37ºC. 67Ga-oxinate complex due to it’s lipophilicity and suitable gamma rays is a suitable cell labeling agent and available for blood stem cell and microorganism studies.
کلیدواژه‌ها English
