نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی علوم زیستی، دانشکده علوم و فنون نوین، دانشگاه تهران

2 پژوهشکده چرخه سوخت هسته ای، پژوهشگاه علوم و فنون هسته ای، تهران، ایران

3 ENTC ، مرکز تحقیقات هسته ای اصفهان، اصفهان

4 گروه علوم جانوری، واحد علوم و تحقیقات،دانشگاه آزاداسلامی ، تهران، ایران

10.24200/nst.2023.1329

چکیده

تمام ترکیبات اورانیم، سمی و رادیواکتیو هستند. سمی بودن این عنصر می‌تواند کشنده باشد. با توجه به سمیت شیمیایی و رادیواکتیویته اورانیم، ضروری است از ورود آن به طبیعت جلوگیری شود. پسماندهای خروجی از مراکز صنعتی- هسته­ای نیز دارای مقادیر مختلفی از اورانیم می­باشد و لذا حذف این عنصر از پساب این مراکز از اهمیت ویژه­ای برخوردار است. از سویی دیگر، نظر به اهمیت این عنصر و محدودیت منابع آن، بازیابی اورانیم از پسماندها، دارای ارزش اقتصادی نیز می­باشد. در پژوهش حاضر، از روش سطح پاسخ (RSM) بر اساس طرح مرکب مرکزی به منظور بررسی و بهینه­سازی پارامترهای مختلف بر فرایند احیای اورانیم توسط باکتری 7Shewanella RCRI در پساب واقعی استفاده گردید. معادله­ی درجه دوم پیشنهاد شده با ضریب همبستگی 94/0=2R از کفایت مناسبی برخوردار بوده و حداکثر راندمان احیای اورانیم توسط 7Shewanella RCRI در شرایط بهینه (pH=5/6-7/5، دمای 63/26 درجه سانتی­گراد و زمان 117 ساعت)، 98% براورد شده است. در گام بعدی به منظور تعامل بین متغیرها، رویه­های سه­بعدی با تقابل pH و دما; دما و زمان;pH  و زمان به دست آمده و در پایان بر اساس بهینه­های پیشنهادی احیای اورانیم در پساب واقعی توسط دو روش XRD و اسپکتروفتومتری مورد بررسی قرار گرفته است. بر اساس نتایج به­دست آمده، باکتری 7Shewanella RCRI می­تواند، به عنوان باکتری با ارزش در احیای زیستی اورانیم از پساب خروجی مراکز صنعتی معرفی گردد. از سوی دیگر استفاده از روش سطح پاسخ می‌توانند درک جامعی از فرایند و مکانیسم احیای زیستی اورانیم توسط باکتری 7Shewanella RCRI و پشتیبانی نظری برای انجام این فرایند ارایه دهد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Optimization of uranium bioreduction by shewanella RCRI7 using Response Surface Design Method (RSM) in industrial waste

نویسندگان [English]

  • Razieh Ghasemi 1
  • Elham Rastkhah 2
  • Sabere Naij 2
  • Touran Rabiee 3
  • Hamzeh Hosseinpour 3
  • Samaneh Naeij 4
  • javad rafiei 2
  • Faezeh Fatemi 2

1 Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran

2 Nuclear Fuel Cycle Research School, Nuclear Science and Technology Research Institute, Tehran, Iran

3 ENTC, Isfahan Nuclear Technology Center, Isfahan, Iran

4 Department of Biochemistry, Faculty of Sciences, Islamic Azad University, Tehran, Iran

چکیده [English]

Uranium compounds are toxic and radioactive. Its poisonous properties can be fatal. So, it is necessary to prevent its excessive entry into nature due to uranium's chemical toxicity and radioactivity. Waste from industrial-nuclear centers also has different amounts of uranium; therefore, the removal of this element from the effluent of these centers is essential. On the other hand, due to the importance and limited resources, uranium recovery from waste has economic value. In the present study, the Response Surface Method (RSM) based on the central composite design was used to evaluate and optimize different parameters affecting the bioremediation process of Shewanella RCRI7 in real waste. The proposed second-order model with a correlation coefficient R2 = 0.94 appropriately predicted the experimental data and the maximum uranium reduction efficiency by Shewanella RCRI7 under optimal conditions (pH = 5.7-6.5, Temperature 26.63 °C and Time 117 hours) was estimated to be about 98%. In the next step to interact between the variables, three-dimensional procedures with pH and temperature; temperature and time; pH and time interactions were obtained; finally, the uranium reduction in real effluent was investigated by XRD and spectrophotometric methods. Based on the results, Shewanella RCRI7 is determined as a valuable candidate for uranium bioreduction processes in the determined industrial wastewater. On the other hand, using the response surface methodology can provide a comprehensive understanding of the process, the mechanism of uranium bioremediation by Shewanella RCRI7, and the theoretical support for this process.

کلیدواژه‌ها [English]

  • Bioremediation
  • Uranium
  • Response surface design method (RSM)
  • Shewanella RCRI7
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