نوع مقاله : مقاله پژوهشی
نویسندگان
پژوهشکده‌ی کشاورزی هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، سازمان انرژی اتمی، صندوق پستی: 1498-31485، کرج - ایران
چکیده
ویروس بیماری تب‌برفکی باعث زیان‌های اقتصادی زیادی در دام‌ها می‌شود. در این تحقیق از ویروس تب‌برفکی سروتایپ 05A-Iran- استفاده شد. پس از تکثیر ویروس در سلول کلیه بچه هامستر پرتوتابی با دزهای مختلف بیم الکترون (10، 20، 25، 30، 35، 40، 45 و 50 کیلوگری) انجام و با استفاده از نرم‌افزار Origin منحنی دز/ پاسخ ترسیم گردید. با استفاده از این منحنی و نتایج آزمون بی‌ضرری و آزمون ثبوت کمپلمان جهت تأیید غیرفعال‌سازی کامل ویروس و بررسی خواص آنتی‌ژنیک ویروس ارزش 10D و دز مطلوب غیرفعال‌سازی ویروس به ترتیب 33/8 و 55 کیلوگری تعیین شد. سپس واکسن پرتوتابی شده و واکسن غیرفعال شده با اتیلن‌ایمین با استفاده از ژل آلومینیم هیدروکسید و ساپونین فرمولاسیون شده و جهت تزریق به خوکچه‌هندی استفاده شدند. تیتر‌آنتی‌بادی خنثی‌کننده و میزان تکثیر لنفوسیت‌های طحال نشان داد که پاسخ‌های ایمنی همورال و سلولی در گروه‌های حیوانی واکسینه شده با هر دو واکسن نسبت به کنترل منفی افزایش معنی‌داری را نشان می‌دهد (05/0(P<. هم‌چنین دز حفاظتی واکسن پرتوتابی شده و واکسن مرسوم در آزمون چلنج به ترتیب 28/6 و 13/6 به دست آمدند که اثر حفاظتی این واکسن‌ها در مدل خوکچه‌هندی را نشان می‌دهند.
کلیدواژه‌ها
عنوان مقاله [English]
Evaluation of the protective effect of inactivated foot and mouth disease subtype A-IRAN-05 using an electron beam in a guinea pig model
نویسندگان [English]
- F. Motamedi Sedeh
- M. Behgar
- S.M. Moosavi
Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, AEOI, P.O.Box: 31485-1498, Karaj - Iran
چکیده [English]
Foot and Mouth Disease Virus (FMDV) can cause a lot of economic losses in livestock. In this research FMDV subtype A-IRAN-05 was used. FMDV was multiplied on BHK21 cells and irradiated by electron beam (10, 20, 25, 30, 35, 40, 45, 50 kGy), then the dose/response curve was drawn by Origin software. D10 value and optimum dose for virus inactivation according to the dose/ response curve and the results of the safety test and Complement Fixation test for the evaluation of antigenic characteristics were obtained at 8.33 and 55 kGy, respectively. Then irradiated vaccine and conventional vaccine were formulated with Aluminum Hydroxide gel and Saponin and used for injection on the guinea pig. The neutralizing antibody titration and splenic lymphocyte proliferation assay were shown a significant increase in humeral and cellular immunity induction in the irradiated vaccine and conventional vaccine to the negative control group (P<0.05). Also, the protective doses of irradiated and conventional vaccines were obtained at 6.28 and 6.13, respectively. It indicated the protective effect of both of the vaccines on a guinea pig.
کلیدواژه‌ها [English]
- Irradiation
- Electron
- Foot
- and mouth disease virus
- Immune response
- Antibody titration
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