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نشان‌دارسازی رزمارینیک اسید Rosmarinic Acid)) پرتودیده با گالیم-67

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی گرگان، صندوق پستی: ۴۹۱۳۸۱۵۷۳۹، گرگان - ایران

2 پژوهشگاه علوم و فنون هسته‌ای، سازمان انرژی اتمی ایران، صندوق پستی: 836-14395، تهران – ایران

چکیده

عصاره گیاه رزماری، 4 برابر آنتی­اکسیدان‌­های مصنوعی مانند BHT و BHA خاصیت آنتی­اکسیدانی دارد. رزمارینیک اسید نه تنها به دلیل خواص آنتی­اکسیدانی قوی مورد توجه است بلکه خصوصیات ضدالتهابی، ضدتوموری، ضدباکتریایی، ضدویروسی و ضدسرطانی را نیز در پژوهش‌­های مختلف نشان داده است. هدف از این تحقیق، بررسی اثرات دما، زمان، pH و غلظت رزمارینیک اسید پرتودیده و نشان‌دارسازی آن با رادیوایزوتوپ گالیم-67 به عنوان یک عامل تصویربرداری با وضوح بالا برای توموگرافی رایانه‌ای تک فوتونی است. در مطالعه حاضر، رزمارینیک اسید تحت تابش گاما با مقادیر 20 و 30 کیلوگری در دو غلظت 1 و 5/0 درصد با رادیوایزوتوپ گالیم-67 تولید شده در سیکلوترون کرج نشان‌دار و راندمان و خلوص رادیوشیمیایی آن‌ها با هم مقایسه شد. شرایط نشان‌دارسازی (از جمله pH، دما، زمان و غلظت) مورد بررسی قرار گرفتند. کنترل کیفی به وسیله کروماتوگرافی لایه نازک (RTLC) انجام گرفت. بر اساس آزمون‌­های صورت گرفته، تیمار بهینه نشان‌دارسازی رزمارینیک اسید پرتو دیده در سطح 30 کیلوگری و غلظت 1 درصد و pH  برابر 5/5-6، در زمان 30 دقیقه در دمای 45 درجه سانتی‌­گراد، بالاترین راندمان و خلوص رادیوشیمیایی 95‌% به‌دست آمد. ضمناً ماده نشان‌دار پایداری خوبی حتی بعد از 12 ساعت از خود نشان داد.

کلیدواژه‌ها

عنوان مقاله [English]

Radiolabeling of gamma-irradiated rosmarinic acid by 67Gallium

نویسندگان [English]

  • N. Damavandi Kamali 1
  • S. Rajabifar 2
  • M. Heidarieh 2

1 Gorgan University of Agricultural Sciences and Natural Resources, P.O.Box: 4913815739, Gorgan - Iran

2 Nuclear Science and Technology Research Institute, AEOI, P.O. Box 14395-836, Tehran - Iran

چکیده [English]

Rosemary plant extract as a natural anti-oxidant is 4 times stronger than synthetic anti-oxidant like BHT and BHA. For this reason, it has been under attention not only for its anti-oxidant properties rather for its anti-inflammatory, anti-tumor, anti-bacterial, and anti-virus properties in different studies. This research investigates the effects of temperature, time, pH, and substance concentration in the labeling of irradiated rosmarinic acid by radioisotope gallium-67 as a high-resolution imaging agent for SPECT imaging. In this study, gamma irradiated rosmarinic acid nanoparticles at 20 kGy and 30 kGy levels in two concentrations of 0.5 and 1% were radiolabeled by gallium-67 radioisotope produced in Karaj cyclotron, and their efficiency and radiochemical purity were compared. Labeling conditions (including pH, temperature, time, and compound concentration) were investigated. Quality control was performed by thin-layer chromatography (RTLC). Resulting from the experiments, 30 kGy level and 1% concentration at 45 °C for 30 minutes at pH = 5.5-6 proved to be the best time for labeling rosemary nanoparticles, and the highest radiochemical purity achieved was 95%; radio conjugate also showed good stability after 12 hours.

کلیدواژه‌ها [English]

  • Gallium-67
  • Radiolabeling
  • Rosmarinic acid
  • Radiochemical purity
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مجله علوم، مهندسی و فناوری هسته‌ای
دوره 44، شماره 4 - شماره پیاپی 106
دی 1402
صفحه 163-169
فایل ها
هم رسانی
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