مجله علوم، مهندسی و فناوری هسته‌ای

اثر حفاظتی ترکیبی ویبریو پاراهمولیتیکوس غیرفعال و ویروس سندروم لکه سفید پرتوتابی شده بر میگو وانامی به دو روش تزریقی و حمامی

نوع مقاله : مقاله پژوهشی

نویسندگان

فرحناز معتمدی سده
رضا روشن

پژوهشکده‌ی کشاورزی هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، سازمان انرژی اتمی، صندوق پستی: 1498-31485، کرج- ایران

https://doi.org/10.24200/nst.2022.1199.1780
چکیده
ویروس سندرم لکه سفید یکی از عوامل عفونت‌زای مهم میگو می‌باشد. در این تحقیق، از نمونه‌های میگو عفونی و جمع‌آوری شده از مزارع میگو استان بوشهر، ویروس سندروم  لکه سفید جداسازی و در همولنف خرچنگ دراز تکثیر و از طریق آزمون PCR وجود ویروس تأیید گردید. تیتراسیون ویروس در پست لاروهای میگو به روش کربر /ml50LD 105.4 محاسبه و غیرفعال‌سازی با بیم الکترون انجام شد. ارزش 10D و دز مطلوب پرتو الکترون برای غیرفعال‌سازی این ویروس به ترتیب 85/1 و 13 کیلوگری به‌دست آمد. ویروس پرتوتابی شده با الکترون به عنوان واکسن- الکترون و باکتری ویبریوپاراهمولیتیکوس پرتوتابی شده به عنوان محرک برای ایمن‌سازی میگو وانامی استفاده شدند. دز حفاظتی 50‌% برای گروه‌های دریافت‌کننده واکسن الکترون، واکسن الکترون همراه با باکتری ویبریو پرتوتابی شده و گروه دریافت‌کننده باکتری ویبریو پرتوتابی شده به تنهایی در روش تزریقی به ترتیب 62/5، 30/6 و 87/2 محاسبه شد. درصد بقا نسبی این سه گروه در روش تزریقی به ترتیب 64‌%، 72‌% و 22‌% و در روش حمامی به ترتیب 75‌%، 85‌% و 5/12‌% محاسبه شد. با توجه به نتایج آزمون مواجهه دو گروه 1 و 2 در مقابل ویروس زنده اثر حفاظتی خوبی نشان دادند. لذا نتیجه‌گیری می‌شود که باکتری ویبریو پاراهمولیتیکوس پرتوتابی شده می‌تواند به عنوان یک محرک ایمنی اثر حفاظتی واکسن- الکترون را در میگوها تقویت نماید. هم‌چنین هر دو روش تزریقی و حمامی برای واکسیناسیون میگوها بدون اختلاف معنی‌دار در درصد بقا نسبی مناسب می‌باشند.

کلیدواژه‌ها

میگو
ویروس سندرم لکه سفید
بیم الکترون
ویبریو پاراهمولیتیکوس
واکسن

عنوان مقاله English

The combined protective effect of inactivated Vibrio Parahemolyticus and irradiated white spot syndrome virus on Litopenaeus vannamei by two routes of administration (injection and immersion)

نویسندگان English

F. Motamedi Sedeh
R. Roshan
Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, AEOI, P.O.Box: 31485-1498, Karaj - Iran
چکیده English

White spot syndrome virus (WSSV) is one of shrimp and other crustaceans' most significant infectious agents. This research isolated WSSV from infected shrimp samples collected from Bushehr’s farms. It was confirmed by PCR and multiplied in Astacus leptodactylus crayfish hemolymph. Titration of WSSV was obtained in postlarvae by the Karber method as 10 5.4 LD50/mL and the virus were inactivated by the electron beam irradiation. The electron beams D10 value and optimum dose was obtained at 1.85 and 13 kGy. Electron beam irradiated WSSV (EBI-WSSV) was used as an electron vaccine to immunize L. vannamei. Gamma-irradiated inactivated Vibrio Parahaemolyticus (GIVP) was used as an immune stimulant. PD50 was calculated 5.62, 6.30 and 2.87 for the injected groups with EBI–WSSV vaccine, EBI-WSSV vaccine+ GIVP and GIVP alone, respectively. The relative percent survival (RPS) values were calculated 64%, 72% and 22% for the EBI-WSSV vaccine, EBI-WSSV+ GIVP and GIVP groups by injection route and 75%, 85% and 12.5% for these three vaccine groups in immersion route, respectively. A significant difference in cumulative mortalities was observed between both vaccination groups (EBI-WSSV and EBI-WSSV+ GIVP), and the GIVP group (P<0.05). Therefore, two vaccine groups 1 and 2 induced productivity responses in shrimp against WSSV infection and GIPV enhanced this response. The conclusion showed the irradiated Vibrio Parahaemolyticus can be used as an immune stimulator and can enhance the protective effect of electron WSSV vaccine. The RPSs in the vaccinated groups by injection and immersion routes are without any significant differences.

کلیدواژه‌ها English

Shrimp
White spot syndrome
Electron beam
Vibrio parahaemolyticus
Vaccine
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